Article (Scientific journals)
Analysis of the diastereoisomers of alliin by HPLC
DETHIER, Bérénice; Laloux, Morgan; Hanon, Emilien et al.
2012In Talanta, 101, p. 447-452
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Keywords :
HPLC; Validation; Porous graphitic carbon; alliin; garlic
Abstract :
[en] Garlic has been known for its therapeutic effects for centuries and is used worldwide as a functional food. The concentration of the active molecules could be enhanced by a better knowledge of their biosynthesis. The precursor of these compounds, alliin (a sulfur amino-acid) has been obtained by chemical synthesis. However, this synthesis route also leads to a diastereoisomer as co-product. This work describes the development of an analytical method which allows the separation and quantification of the two diastereoisomers in order to determine in which proportion the natural form can be produced. The HPLC method which was optimized and validated by accuracy profile exploits an original stationary phase consisting of porous graphitic carbon (PGC). Furthermore, the developped method was used to separate the diastereoisomers of methiin, another garlic cysteine sulfoxide, and to analyse and aqueous extract of garlic. The ability to quantify the amount of natural alliin is valuable for further work on garlic molecules and their application for health protection.
Disciplines :
Chemistry
Author, co-author :
DETHIER, Bérénice ;  Université de Liège - ULiège > Chimie et bio-industries > Chimie générale et organique
Laloux, Morgan ;  Université de Liège - ULiège > Chimie et bio-industries > Chimie générale et organique
Hanon, Emilien
Nott, Katherine ;  Université de Liège - ULiège > Chimie et bio-industries > Chimie générale et organique
Heuskin, Stéphanie ;  Université de Liège - ULiège > Chimie et bio-industries > Analyse, qual. et risques - Labo. de Chimie analytique
Wathelet, Jean-Paul ;  Université de Liège - ULiège > Chimie et bio-industries > Chimie générale et organique
Language :
English
Title :
Analysis of the diastereoisomers of alliin by HPLC
Alternative titles :
[fr] Analyse des diastéréoisomères de l'alliine par HPLC
Publication date :
15 November 2012
Journal title :
Talanta
ISSN :
0039-9140
eISSN :
1873-3573
Publisher :
Elsevier Science, Amsterdam, Netherlands
Volume :
101
Pages :
447-452
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 18 October 2012

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