|Reference : USE OF FLUORESCENTLY LABELLED PROTOZOANS TO INVESTIGATE THE PREDATION OF ROTIFERS ON ...|
|Scientific congresses and symposiums : Unpublished conference|
|Life sciences : Environmental sciences & ecology|
|USE OF FLUORESCENTLY LABELLED PROTOZOANS TO INVESTIGATE THE PREDATION OF ROTIFERS ON PROTOZOOPLANKTON|
|Joaquim-Justo, Célia [Université de Liège - ULg > Département de Biologie, Ecologie et Evolution > Ecologie animale et écotoxicologie >]|
|Detry, Cédric [Université de Liège - ULg > Services généraux (Faculté de médecine) > Service administratif de la Faculté (Médecine) >]|
|Thomé, Jean-Pierre [Université de Liège - ULg > Département de Biologie, Ecologie et Evolution > Ecologie animale et écotoxicologie >]|
|IX International Rotifer Symposium|
|du 16 au 23 janvier 2000|
|[en] The importance of trophic relationships between rotifers and protozoans in rivers are not yet well known, but as some authors have shown during the last decade, the predation exerted by rotifers on these organisms might be of importance in the transfer of energy from the pico- and nanoplankton to the metazooplankton.
Fluorescent polycarbonate beads of 0.45 µm in diameter were used to label the ciliate Tetrahymena pyriformis.
Different combinations of labelling times and concentrations of microspheres were tested to achieve an optimal labelling of the ciliates (i.e. a maximal proportion of individuals having ingested the same number of microspheres). Live labelled Tetrahymena pyriformis were used to determine both the gut passage time and the ingestion rate of the rotifer Brachionus calyciflorus. The ingestion rates measured with relatively high densities of ciliates (from 30 to 500 cells.ml-1) were on average of 30 cells.ind-1.day-1. The ingestion rates yielded by this method were compared with ingestion rates measured by assessing the disappearance of the ciliates in experimental media during 5-6 hour incubations. Results from both methods were comparable but the use of fluorescently labelled prey items produced less variable results.
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