Reference : Myeloperoxidase as an indicator of endometritis in the mare: preliminary results
Scientific congresses and symposiums : Paper published in a journal
Life sciences : Veterinary medicine & animal health
http://hdl.handle.net/2268/132071
Myeloperoxidase as an indicator of endometritis in the mare: preliminary results
English
Parrilla Hernandez, Sonia [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Département clinique des animaux de compagnie et des équidés >]
Franck, Thierry mailto [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux >]
Ponthier, Jérôme mailto [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux >]
Mottart, Evelyne mailto [Cereq > > > >]
Deleuze, Stefan mailto [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Obstét. et path. de la reprod. des anim. de comp. et équidés >]
Aug-2012
Reproduction in Domestic Animals
Blackwell Publishing
47
suppl 5
65
Yes (verified by ORBi)
International
0936-6768
1439-0531
Berlin
Germany
16th congress of the European Society For Domestic Animals Reproduction
31/08/2012 au 01/09/2012
European Society For Domestic Animals Reproduction
Dublin
Ireland
[en] Equine ; Mare ; Endometritis ; Myeloperoxidase ; Diagnosis
[en] Diagnosis of endometritis in the mare is routinely based on the presence of polymorphonuclear cells (PMNs) on endometrial smears. Studies show a relation between PMNs and myeloperoxidase (MPO), an enzyme released by PMNs during degranulation or after cell lysis, in many fluids and tissues. The aims of this study were to assess the presence and concentration of MPO in the mare’s uterus, and to investigate its relation with PMNs. Thirty-six cycles from 28 mares (ages ranging from 6 to 22 years) were used. Endometrial cytological samples were obtained with a small volume uterine flush and either a uterine cotton swab or a cytobrush, when a follicle >35 mm was observed by ultrasound. The smears were stained with Diff-QuickÒ and one or more PMNs per field (400·) was diagnosed as endometritis. The supernatant of the flushes was used to measure MPO concentration with a specific equine MPO ELISA assay. Our results showed the presence of MPO in the equine uterus during oestrus (mean = 2839 + 2785). MPO concentrations were signifi- cantly (p < 0.05) higher in samples with positive cytological results. Occasionally, some samples with negative cytological results showed high MPO concentration, but the opposite was never observed. Clinical signs of endometritis are not always present, or they may be delayed. An early diagnostic improves the success of treatment. Our results show that high quantities of MPO in endometrial samples indicate the presence of PMNs. Further studies are needed to determine if MPO concentration could be routinely used as a tool of early detection of endometritis.
Centre Européen du Cheval - LINALUX Mont le Soie ; Centre de l’Oxygène, Recherche et Développement - CORD
Centre Européen du Cheval - LINALUX Mont le Soie ; ULg - FMV - Clinique Equine
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/132071

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