|Reference : Study of the Boettcher cells along their development: Junctions and expression of the ur...|
|Scientific congresses and symposiums : Poster|
|Life sciences : Anatomy (cytology, histology, embryology...) & physiology|
|Study of the Boettcher cells along their development: Junctions and expression of the urea-transporter B (UT-B)|
|Cloes, Marie [Université de Liège - ULg > Département des sciences de la vie > Biologie cellulaire >]|
|Renson, Thomas [Université de Liège - ULg > Département des Sciences de la Vie > Biologie cellulaire > >]|
|Johnen, Nicolas [Université de Liège - ULg > Département des sciences de la vie > Biologie cellulaire >]|
|Francart, Marie-Emilie [Université de Liège - ULg > > > GIGA - Utilisateurs machines]|
|Thelen, Nicolas [Université de Liège - ULg > Département des sciences de la vie > Biologie cellulaire >]|
|Thiry, Marc [Université de Liège - ULg > Département des sciences de la vie > Biologie cellulaire >]|
|Inner Ear Biology 2012 Workshop and Symposium|
|du 29 septembre 2012 au 02 octobre 2012|
|[en] Boettcher ; UT-B|
|[en] The Boettcher cells (BC) lie on the sensory epithelium of the cochlea. Their function has never been clearly defined. However it has been suggested that they may influence the ionic composition of the fluids of the inner ear, which play a central role in the conduction of the sensory information. In this context the compartimentating function of the BC around and after the onset of hearing may influence the subsequent refining of hearing.
We collected ultrastructural and immunohistological data during the final maturation stage of the sensory epithelium. In particular the cell junctions were investigated to clarify the compartimentating function of the BC at early stages. As a potential actor in the ion flow in the sensory epithelium, the urea transporter-B (UT-B) was also immunolocalised during the development of the BC.
At the mature stage (P25) the BC are linked to the adjacent cells by numerous adherens and non-adherens junctions. They rest on a basilar membrane to which they are attached by hemidesmosomes. They typically exhibit large basolateral interdigitations. We found that, at the 8th postnatal day, the BC are separated from the neighbouring cells by wide spaces entered by scarce cytoplasmic extensions. These spaces are interrupted by areas of close contact, where adherens and non-adherens junctions may be found. Thus, although there seems to be fewer interdigitations at P8, gap junctions probably still allow easy cell-to-cell exchanges. Moreover non-adherens junctions can systematically be identified apically. Although it was impossible to differenciate tight and gap junctions without specific labeling, we postulate that these non-adherens junctions correspond to tight junctions and seal the apex of the BC. This feature is necessary to enable the control of the ion concentrations surrounding the sensory epithelium. We also found that UT-B, known for water and urea transport in red blood cells, is present in the membranes of the BC from P12 (the earliest stage tested) to P25. Thus UT-B may play a role in the regulation of the ionic concentrations of the inner ear fluids.
|Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS|
|Researchers ; Professionals|
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