Article (Scientific journals)
Mesenchymal stem cells and neural crest stem cells from adult bone marrow: characterization of their surprising similarities and differences.
Wislet, Sabine; Laudet, Emerence; Neirinckx, Virginie et al.
2012In Cellular and Molecular Life Sciences, 69 (15), p. 2593-2608
Peer Reviewed verified by ORBi
 

Files


Full Text
CMLS 2012.pdf
Publisher postprint (1.39 MB)
Request a copy
Annexes
Retraction Note 2012.pdf
(301.13 kB)
Download

All documents in ORBi are protected by a user license.

Send to



Details



Abstract :
[en] The generation of neuronal cells from stem cells obtained from adult bone marrow is of significant clinical interest in order to design new cell therapy protocols for several neurological disorders. The recent identification in adult bone marrow of stem cells derived from the neural crest stem cells (NCSC) might explain the neuronal phenotypic plasticity shown by bone marrow cells. However, little information is available about the nature of these cells compared to mesenchymal stem cells (MSC), including their similarities and differences. In this paper, using transcriptomic as well as proteomic technologies, we compared NCSC to MSC and stromal nestin-positive cells, all of them isolated from adult bone marrow. We demonstrated that the nestin-positive cell population, which was the first to be described as able to differentiate into functional neurons, was a mixed population of NCSC and MSC. More interestingly, we demonstrated that MSC shared with NCSC the same ability to truly differentiate into Tuj1-positive cells when co-cultivated with paraformaldehyde-fixed cerebellar granule neurons. Altogether, those results suggest that both NCSC and MSC can be considered as important tools for cellular therapies in order to replace neurons in various neurological diseases.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Wislet, Sabine  ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie générales, et biochimie humaine
Laudet, Emerence ;  Université de Liège - ULiège > GIGA - Neurosciences
Neirinckx, Virginie ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie
Alix, Philippe ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Pharmacologie
Leprince, Pierre ;  Université de Liège - ULiège > GIGA - Neurosciences
glejzer, Aneta
Poulet, Christophe  ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > GIGA-R : Génétique humaine
Hennuy, Benoît ;  Université de Liège - ULiège > GIGA-Management : Plate-forme transcriptomique
Sommer, Lukas
Shakhova, Olga
Rogister, Bernard  ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie générales, et biochimie humaine
Language :
English
Title :
Mesenchymal stem cells and neural crest stem cells from adult bone marrow: characterization of their surprising similarities and differences.
Publication date :
August 2012
Journal title :
Cellular and Molecular Life Sciences
ISSN :
1420-682X
eISSN :
1420-9071
Publisher :
Birkhäuser, Basel, Switzerland
Volume :
69
Issue :
15
Pages :
2593-2608
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 09 October 2012

Statistics


Number of views
120 (26 by ULiège)
Number of downloads
7 (4 by ULiège)

Scopus citations®
 
64
Scopus citations®
without self-citations
55
OpenCitations
 
60

Bibliography


Similar publications



Contact ORBi