Reference : Increased hypoxia-inducible factor 1alpha expression in lung cells of horses with recurr...
Scientific journals : Article
Life sciences : Veterinary medicine & animal health
http://hdl.handle.net/2268/131669
Increased hypoxia-inducible factor 1alpha expression in lung cells of horses with recurrent airway obstruction.
English
Toussaint, Marie mailto [Université de Liège - ULg > Département de sciences fonctionnelles > GIGA-R : Biochimie et biologie moléculaire >]
Fievez, Laurence mailto [Université de Liège - ULg > Département de sciences fonctionnelles > GIGA-R : Biochimie et biologie moléculaire >]
Desmet, Christophe mailto [Université de Liège - ULg > Département de sciences fonctionnelles > Physiologie >]
Pirottin, Dimitri mailto [Université de Liège - ULg > Département de sciences fonctionnelles > Département de sciences fonctionnelles >]
Farnir, Frédéric mailto [Université de Liège - ULg > Département de productions animales > Biostatistiques et bioinformatique appliquées aux sc. vétér. >]
Bureau, Fabrice mailto [Université de Liège - ULg > Département de sciences fonctionnelles > GIGA-R : Biochimie et biologie moléculaire >]
Lekeux, Pierre mailto [Université de Liège - ULg > Département de sciences fonctionnelles > Physiologie >]
2012
BMC Veterinary Research
BioMed Central
8
1
64
Yes (verified by ORBi)
International
1746-6148
[en] Hypoxia inductible transcription factor-1 ; Recurrent airway obstruction ; Inflammation ; Lung ; Horse
[en] ABSTRACT: BACKGROUND: Recurrent airway obstruction (RAO, also known as equine heaves) is an inflammatory condition caused by exposure of susceptible horses to organic dusts in hay. The immunological processes responsible for the development and the persistence of airway inflammation are still largely unknown. Hypoxia-inducible factor (Hif) is mainly known as a major regulator of energy homeostasis and cellular adaptation to hypoxia. More recently however, Hif also emerged as an essential regulator of innate immune responses. Here, we aimed at investigating the potential involvement of Hif1-alpha in myeloid cells in horse with recurrent airway obstruction. RESULTS: In vitro, we observed that Hif is expressed in equine myeloid cells after hay dust stimulation and regulates genes such as tumor necrosis factor alpha (TNF-alpha), interleukin-8 (IL-8) and vascular endothelial growth factor A (VEGF-A). We further showed in vivo that airway challenge with hay dust upregulated Hif1-alpha mRNA expression in myeloid cells from the bronchoalveolar lavage fluid (BALF) of healthy and RAO-affected horses, with a more pronounced effect in cells from RAO-affected horses. Finally, Hif1-alpha mRNA expression in BALF cells from challenged horses correlated positively with lung dysfunction. CONCLUSION: Taken together, our results suggest an important role for Hif1-alpha in myeloid cells during hay dust-induced inflammation in horses with RAO. We therefore propose that future research aiming at functional inactivation of Hif1 in lung myeloid cells could open new therapeutic perspectives for RAO.
Researchers ; Professionals
http://hdl.handle.net/2268/131669

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