Article (Scientific journals)
Increased hypoxia-inducible factor 1alpha expression in lung cells of horses with recurrent airway obstruction.
Toussaint, Marie; Fievez, Laurence; Desmet, Christophe et al.
2012In BMC Veterinary Research, 8 (1), p. 64
Peer Reviewed verified by ORBi
 

Files


Full Text
ToussaintM_BMCVetRes_2012.pdf
Publisher postprint (639.96 kB)
Download

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
Hypoxia inductible transcription factor-1; Recurrent airway obstruction; Inflammation; Lung; Horse
Abstract :
[en] ABSTRACT: BACKGROUND: Recurrent airway obstruction (RAO, also known as equine heaves) is an inflammatory condition caused by exposure of susceptible horses to organic dusts in hay. The immunological processes responsible for the development and the persistence of airway inflammation are still largely unknown. Hypoxia-inducible factor (Hif) is mainly known as a major regulator of energy homeostasis and cellular adaptation to hypoxia. More recently however, Hif also emerged as an essential regulator of innate immune responses. Here, we aimed at investigating the potential involvement of Hif1-alpha in myeloid cells in horse with recurrent airway obstruction. RESULTS: In vitro, we observed that Hif is expressed in equine myeloid cells after hay dust stimulation and regulates genes such as tumor necrosis factor alpha (TNF-alpha), interleukin-8 (IL-8) and vascular endothelial growth factor A (VEGF-A). We further showed in vivo that airway challenge with hay dust upregulated Hif1-alpha mRNA expression in myeloid cells from the bronchoalveolar lavage fluid (BALF) of healthy and RAO-affected horses, with a more pronounced effect in cells from RAO-affected horses. Finally, Hif1-alpha mRNA expression in BALF cells from challenged horses correlated positively with lung dysfunction. CONCLUSION: Taken together, our results suggest an important role for Hif1-alpha in myeloid cells during hay dust-induced inflammation in horses with RAO. We therefore propose that future research aiming at functional inactivation of Hif1 in lung myeloid cells could open new therapeutic perspectives for RAO.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Toussaint, Marie ;  Université de Liège - ULiège > Département de sciences fonctionnelles > GIGA-R : Biochimie et biologie moléculaire
Fievez, Laurence ;  Université de Liège - ULiège > Département de sciences fonctionnelles > GIGA-R : Biochimie et biologie moléculaire
Desmet, Christophe  ;  Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie
Pirottin, Dimitri ;  Université de Liège - ULiège > Département de sciences fonctionnelles > Département de sciences fonctionnelles
Farnir, Frédéric  ;  Université de Liège - ULiège > Département de productions animales > Biostatistiques et bioinformatique appliquées aux sc. vétér.
Bureau, Fabrice ;  Université de Liège - ULiège > Département de sciences fonctionnelles > GIGA-R : Biochimie et biologie moléculaire
Lekeux, Pierre ;  Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie
Language :
English
Title :
Increased hypoxia-inducible factor 1alpha expression in lung cells of horses with recurrent airway obstruction.
Publication date :
2012
Journal title :
BMC Veterinary Research
eISSN :
1746-6148
Publisher :
BioMed Central
Volume :
8
Issue :
1
Pages :
64
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 05 October 2012

Statistics


Number of views
97 (4 by ULiège)
Number of downloads
70 (0 by ULiège)

Scopus citations®
 
8
Scopus citations®
without self-citations
8
OpenCitations
 
6

Bibliography


Similar publications



Contact ORBi