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Poster (Scientific congresses and symposiums)
UNFOLDING SYNTHETIC PEPTIDES BY AFM AT THE SINGLE-MOLECULE LEVEL
Willet, Nicolas; Hinterdorfer, Peter; Lecommandoux, Sébastien et al.
2012Sofia School of Protein Science: Structure and dynamics of biological macromolecules
 

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Keywords :
AFM; Force spectroscopy; Folding; Peptide; Single-Molecule; Conformation
Abstract :
[en] The aim of this study is to investigate the mechanochemical behavior of homopolypeptides able to change their conformation is a stimuli-responsive way. The peptidic secondary structures were studied in detail by atomic force microscopy (AFM) at the single-molecule level. Synthetic copolymers containing a polypeptide block were prepared by N-carboxyanhydride amino acid ring-opening polymerization. The polymer chains were grafted as a dilute brush onto gold surfaces and their mechanochemical behavior was then studied by AFM single-molecule force spectroscopy (SMFS). The investigated polypeptide blocks were based on poly(L-glutamic acid), which undergoes a transition from alpha-helix to random coil. This can be induced by external stimuli (pH, ionic strength, temperature) or simply by applying a force. We were able to study the mechanically driven unfolding of the peptide by stretching-release cycles of the biomacromolecule. Stretching the helical peptide resulted in original features in the force-distance traces. Plateaus that are specific for the helical conformation were detected, quantified and discussed. Pulling-relaxing SMFS experiments will eventually lead to a better understanding of the force-induced unfolding of an alpha-helix and the reversibility of the phenomenon.
Disciplines :
Biochemistry, biophysics & molecular biology
Chemistry
Physics
Physical, chemical, mathematical & earth Sciences: Multidisciplinary, general & others
Author, co-author :
Willet, Nicolas ;  Université de Liège - ULiège > Département de chimie (sciences) > Département de chimie (sciences)
Hinterdorfer, Peter;  Johannes Kepler University Linz
Lecommandoux, Sébastien;  Université Bordeaux 1
Duwez, Anne-Sophie  ;  Université de Liège - ULiège > Département de chimie (sciences) > Nano-chimie et systèmes moléculaires
Language :
English
Title :
UNFOLDING SYNTHETIC PEPTIDES BY AFM AT THE SINGLE-MOLECULE LEVEL
Publication date :
September 2012
Event name :
Sofia School of Protein Science: Structure and dynamics of biological macromolecules
Event organizer :
FEBS (Federation of European Biochemical Societies)
Event place :
Sofia, Bulgaria
Event date :
9-14 September 2012
Audience :
International
Additional URL :
Available on ORBi :
since 03 October 2012

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