Reference : Discrimination of Isobaric Leu/Ile Residues by MALDI In-source Decay Mass Spectrometry
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Physical, chemical, mathematical & earth Sciences : Chemistry
http://hdl.handle.net/2268/131140
Discrimination of Isobaric Leu/Ile Residues by MALDI In-source Decay Mass Spectrometry
English
Asakawa, Daiki [> >]
Smargiasso, Nicolas mailto [Université de Liège - ULg > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.) >]
De Pauw, Edwin mailto [Université de Liège - ULg > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.) >]
Feb-2013
Journal of the American Society for Mass Spectrometry
Spinger
24
2
297-300
Yes (verified by ORBi)
International
1044-0305
New York
USA
[en] Top down sequencing ; MALDI ISD ; LEU/ILEU
[en] MALDI in-source decay (ISD) has been used for the top-down sequencing of proteins.
The use of 1,5-diaminonapthalene (1,5-DAN) gave strong intensity of w ions, which are
informative fragments and can be helpful for the distinction of the isobaric amino acids,
Leu and Ile. Our data suggests that the w fragments are formed from z* radical
fragment by unimolecular dissociation and high abundance of w ions in MALDI-ISD
with 1,5-DAN can be understood as resulting from the low collision rate in the MALDI
plume. The MALDI-ISD with 1,5-DAN could be a useful method for the top-down
sequencing of proteins including discrimination of Leu and Ile near the C-terminal end.
Giga-Systems Biology and Chemical Biology
Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS ; Japan Society for the Promotion of Science
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/131140
10.1007/s13361-012-0523-7
http://link.springer.com/article/10.1007%2Fs13361-012-0523-7
FP7 ; 278346 - VENOMICS - High-throughput peptidomics and transcriptomics of animal venoms for discovery of novel therapeutic peptides and innovative drug development

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