Reference : Microscopic study of equine oocyte maturation within quaternary follicles
Scientific journals : Article
Life sciences : Veterinary medicine & animal health
Life sciences : Anatomy (cytology, histology, embryology...) & physiology
http://hdl.handle.net/2268/13063
Microscopic study of equine oocyte maturation within quaternary follicles
English
[fr] Etude microscopique de la maturation de l'ovocyte équin au sein des follicules quaternaires
Ponthier, Jérôme mailto [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Département clinique des animaux de compagnie et des équidés >]
Beckers, Jean-François mailto [Université de Liège - ULg > Département de sciences fonctionnelles > Physiologie de la reproduction >]
Deleuze, Stefan mailto [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux - Département clinique des animaux de compagnie et des équidés >]
2009
Comptes Rendus de l'Académie Bulgare des Sciences = Proceeding of the Bulgarian Academy of Sciences
62
4
491-498
Yes (verified by ORBi)
International
1310-1331
Bulgaria
[en] horse ; oocyte ; IVM ; microscopy
[en] In various species a relationship between oocyte maturity and morphological characteristics of oocytes has been established. To our knowledge, little
attention has been paid to the immature oocyte aspect under light microscopy in the equine. The aim of the study is to give a general description and analysis
of the microscopic parameters of immature oocytes.
Follicles from abattoir ovaries are punctured after measurement of their diameter and their cumulus-oocyte complexes are recovered. Different characters
such as oocyte diameter, cumulus aspect, granulosity and polarity of ooplasm are observed under light microscopy.
No correlation between the estral activity, follicle maturity, cumulus cells aspect, polarity and granulosity of ooplasm was observed. The thickness of zona pellucida differs between oocytes with polar or nonpolar ooplasm. No correlation was observed between the follicle and oocyte diameter and none of the other studied parameters showed an influence on the oocyte diameter.
Results show that no character of the observed oocytes under light microscopy can be related to the follicular origin or to any other character. Further studies have to be done to compare these immature oocytes with mature ones and to find the ultrastructural origin of the characters observed under light microscopy.
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/13063
The scientific paper is presented on the 1st Conference of the Balkan Network for the Animal
Reproduction Biotechnology within pro ject 4-01-2008 under the European programme “Research
Cooperation and Networking between Austria and South Eastern Europe” supported by the
Austrian Science and Research Liaison Offices.

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