Reference : Comparative enantioseparation of talinolol in aqueous and non-aqueous capillary elect...
Scientific journals : Article
Human health sciences : Pharmacy, pharmacology & toxicology
http://hdl.handle.net/2268/130566
Comparative enantioseparation of talinolol in aqueous and non-aqueous capillary electrophoresis and study of related selector-selectand interactions by nuclear magnetic resonance spectroscopy.
English
Chankvetadze, Lali [> >]
Servais, Anne-Catherine mailto [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
Fillet, Marianne mailto [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
Salgado, Antonio [> >]
Crommen, Jacques mailto [Université de Liège - ULg > Département de pharmacie > Département de pharmacie >]
Chankvetadze, Bezhan [> >]
2012
Journal of Chromatography. A
1267
206-16
Yes (verified by ORBi)
International
0021-9673
1873-3778
[en] Aqueous and non-aqueous capillary electrophoresis ; Nuclear magnetic resonance spectroscopy ; Enantioseparations ; Cyclodextrins ; Reversal of enantiomer migration order
[en] The enantiomers of the chiral beta-blocker drug talinolol were separated with two single component sulfated beta-cyclodextrin (CD) derivatives, namely heptakis (2,3-di-O-methyl-6-sulfo)-beta-CD) (HDMS-beta-CD) and heptakis (2,3-di-O-acetyl-6-sulfo)-beta-CD) (HDAS-beta-CD), in aqueous and non-aqueous capillary electrophoresis (CE). The enantiomer affinity pattern of talinolol toward these two CDs was opposite in both aqueous and non-aqueous CE. However, the enantiomer affinity pattern for a given CD derivative did not change when aqueous buffer was replaced with non-aqueous background electrolyte. The structures of the analyte-selector complexes in both, aqueous and non-aqueous electrolytes were studied using rotating frame nuclear Overhauser effect (ROESY) NMR spectroscopy. Inclusion complex formation between the enantiomers of talinolol and HDAS-beta-CD was confirmed in aqueous buffer, while the complex between the enantiomers of talinolol and HDMS-beta-CD was of the external type. The complex of the talinolol enantiomers with HDAS-beta-CD in non-aqueous electrolyte was also of the external type. In spite of external complex formation excellent separation of the enantiomers was observed in non-aqueous CE.
http://hdl.handle.net/2268/130566
Copyright (c) 2012 Elsevier B.V. All rights reserved.

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