Article (Scientific journals)
Differential expression of two somatostatin genes during zebrafish embryonic development
Devos, Nathalie; Deflorian, Gianluca; Biemar, Frédéric et al.
2002In Mechanisms of Development, 115 (1-2), p. 133-7
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Keywords :
Amino Acid Sequence; Animals; Base Sequence; Brain/embryology/metabolism; Central Nervous System/embryology/*metabolism; DNA, Complementary; *Gene Expression; Gene Expression Profiling; Humans; Molecular Sequence Data; Pancreas/embryology/*metabolism; Phylogeny; Protein Precursors/classification/*genetics; Proteins/genetics; *Repressor Proteins; Sequence Homology, Amino Acid; Somatostatin/classification/*genetics; Zebrafish/embryology/*genetics; Zebrafish Proteins
Abstract :
[en] We have identified the cDNAs of two new zebrafish preprosomatostatins, PPSS1 and PPSS3, in addition to the previously cloned PPSS2 (Argenton et al., 1999). PPSS1 is the orthologue of mammalian PPSSs, with a conserved C-terminal SS-14 sequence, PPSS2 is a divergent SS precursor and PPSS3 is a cortistatin-like prohormone. Using whole-mount in situ hybridisation, we have analysed the expression of PPSS1 and PPSS2 in zebrafish embryos up to 5 days post fertilisation. PPSS1 was expressed in the developing pancreas and central nervous system (CNS), whereas PPSS2 expression was exclusively pancreatic. In the CNS, PPSS1 was detected in several areas, in particular in the vagal motor nucleus and in cells that pioneer the tract of the postoptic commissure. PPSS1 was also expressed transiently in the telencephalon and spinal motor neurons. In all areas but the telencephalon PPSS1 was coexpressed with islet-1.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Devos, Nathalie
Deflorian, Gianluca;  Università di Padova > Dipartimento di Biologia
Biemar, Frédéric
Bortolussi, Marino;  Università di Padova > Dipartimento di Biologia
Martial, Joseph ;  Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Peers, Bernard ;  Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Argenton, Francesco;  Università di Padova > Dipartimento di Biologia
Language :
English
Title :
Differential expression of two somatostatin genes during zebrafish embryonic development
Publication date :
2002
Journal title :
Mechanisms of Development
ISSN :
0925-4773
eISSN :
1872-6356
Publisher :
Elsevier, Limerick, Ireland
Volume :
115
Issue :
1-2
Pages :
133-7
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 23 May 2009

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