Reference : Fast-GC quantifacation of harmonine, the major defense alkaloid of the multicolored Asia...
Scientific journals : Article
Physical, chemical, mathematical & earth Sciences : Chemistry
Life sciences : Entomology & pest control
http://hdl.handle.net/2268/130461
Fast-GC quantifacation of harmonine, the major defense alkaloid of the multicolored Asian ladybird Harmonia axyridis (Pallas)
English
Fischer, Christophe mailto [Université de Liège - ULg > Chimie et bio-industries > Analyse, qual. et risques - Labo. de Chimie analytique >]
Laurent, Pascal mailto [Université de Liège - ULg > Chimie et bio-industries > Chimie biologique industrielle >]
Heuskin, Stéphanie mailto [Université de Liège - ULg > Chimie et bio-industries > Analyse, qual. et risques - Labo. de Chimie analytique >]
Durieux, Delphine [Université de Liège - ULg > Sciences agronomiques > Entomologie fonctionnelle et évolutive >]
Haubruge, Eric mailto [Université de Liège - ULg > Services administratifs généraux > Vice-Recteur de Gembloux Agro Bio Tech >]
Verheggen, François mailto [Université de Liège - ULg > Sciences agronomiques > Entomologie fonctionnelle et évolutive >]
Lognay, Georges mailto [Université de Liège - ULg > Chimie et bio-industries > Analyse, qual. et risques - Labo. de Chimie analytique >]
Jan-2012
American Journal of Analytical Chemistry
3
Yes (verified by ORBi)
International
2156-8251
[en] alkaloid ; harmonine ; Harmonia axyridis ; Fast-GC ; validation
[en] These last few years the multicolored Asian ladybird, Harmonia axyridis (Pallas), previously introduced in Europe and North America as a biological control agent, has swiftly spread out on those territories and turns out to be a pest in several ways. In order to improve understanding of several key points of the biology of this ladybird, a novel fast-GC method of quantification of harmonine, the main defense alkaloid of H. axyridis, has been designed and validated using the accuracy profile concept for concentrations ranging from 13 to 264 µg/individual. This method allows quantifying harmonine in a single insect with a maximal error risk of 20%. For ladybirds collected in spring, mean harmonine concentration observed was 106.6 µg/individual with a very high standard deviation of 80.2 µg/individual, which can easily be explained by the strong asymmetry of the results distribution. Males and females seem equally chemically protected as the harmonine concentration did not significantly differ between sexes. This method is currently used to improve our comprehension of several key points of H. axyridis biology including aggregation behavior and aposematism.
Fonds pour la formation à la Recherche dans l'Industrie et dans l'Agriculture (Communauté française de Belgique) - FRIA
Researchers
http://hdl.handle.net/2268/130461
10.4236/ajac.2012.31010
http://www.scirp.org/journal/PaperInformation.aspx?paperID=16793

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