ORBi: Kouamé-Sina Sylvie Mireille - Diversity, phylogenetic relationship and antibacterial potential of Bifidobacterium species isolated from raw milk production chain in Abidjan (Côte d'Ivoire)

Reference : Diversity, phylogenetic relationship and antibacterial potential of Bifidobacterium spec...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Microbiology
	To cite this reference:	http://hdl.handle.net/2268/130315

Title :	Diversity, phylogenetic relationship and antibacterial potential of Bifidobacterium species isolated from raw milk production chain in Abidjan (Côte d'Ivoire)
Language :	English
Author, co-author :	Kouamé-Sina, Sylvie Mireille [Centre Suisse de Recherches Scientifiques en Côte d’Ivoire, 01 BP 1303 Abidjan 01. Côte d’Ivoire. > > > >]
	Dadié, Adjéhi [University of Abobo-Adjamé, 02 BP 801 Abidjan 02, Côte d’Ivoire. > Faculty of Food Sciences and Technology, > Laboratory of Biotechnology and Food Microbiology, > >]
	Makita, Kohei [International Livestock Research Institute ILRI, Nairobi, P. O. Box 30709 Nairobi, Kenya. > > > >]
	Grace, Delia [International Livestock Research Institute ILRI, Nairobi, P. O. Box 30709 Nairobi, Kenya. > > > >]
	Dje, Marcellin [University of Abobo-Adjamé, 02 BP 801 Abidjan 02, Côte d’Ivoire. > Faculty of Food Sciences and Technology, > Laboratory of Biotechnology and Food Microbiology, > >]
	Taminiau, Bernard [Université de Liège - ULg > Département de sciences des denrées alimentaires > Microbiologie des denrées alimentaires >]
	Daube, Georges [Université de Liège - ULg > Département de sciences des denrées alimentaires > Microbiologie des denrées alimentaires >]
	Bonfoh, Bassirou [Centre Suisse de Recherches Scientifiques en Côte d’Ivoire, 01 BP 1303 Abidjan 01. Côte d’Ivoire. > > > >]
Publication date :	2011
Journal title :	African Journal of Microbiology Research [=AJMR]
Publisher :	Academic Journals
Volume :	5
Issue/season :	21
Pages :	3394-3403
Peer reviewed :	Yes (verified by ORBi)
Audience :	International
ISSN :	1996-0808
City :	Lagos
Country :	Nigeria
Keywords :	[en] Bifidobacterium ; Milk products
Abstract :	[en] The local dairy commodity, from farm to retail point, is informal and often escapes safety surveillance and results in high contamination of local milk by pathogens. The objective of this study was to determine the biodiversity of Bifidobacterium species in the informal dairy production chain in Abidjan and evaluate their potential antibacterial activity against pathogens. Bifidobacterium species were identified after sequencing of hsp60 genes. Results showed that Bifidobacterium were present in 9% of samples. Milkers' hands (14%) and cows’s udders (14%) were the most contaminated with Bifidobacterium. These isolates belong to five different species. Most Bifidobacterium isolated are Bifidobacterium minimum (53%) and Bifidobacterium pseudolongum subsp. Globosum (24.4%). The other strains are composed of one strain of Bifidobacterium thermophilum, Bifidobacterium thermacidophilum subsp. suis and Bifidobacterium magnum. The isolated Bifidobacterium species have antibacterial activities that are not related to bacteriocins production, but to organic acids production (65%), which exert in vitro inhibitory action against Listeria monocytogenes, Salmonella hadar and Salmonella typhimurium, Staphylococcus aureus, Escherichia coli O27 and Escherichia coli O157H7. However, ensuring milk safety along the local milk production chain requires implementation of good hygiene practices together with adapted technology, such as fermentation.
Funders :	Agence universitaire de la francophonie
Name of the research project :	E/4488-1
Target :	Researchers ; Professionals ; Students
Permalink :	http://hdl.handle.net/2268/130315

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