Reference : Collision induced dissociation of deuterium enriched protonated 2'-deoxyguanosine
Scientific journals : Article
Physical, chemical, mathematical & earth Sciences : Chemistry
http://hdl.handle.net/2268/13021
Collision induced dissociation of deuterium enriched protonated 2'-deoxyguanosine
English
Greisch, Jean-François mailto [Université de Liège - ULg > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.) >]
Leyh, Bernard mailto [Université de Liège - ULg > Département de chimie (sciences) > Laboratoire de dynamique moléculaire >]
De Pauw, Edwin mailto [Université de Liège - ULg > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.) >]
Jan-2009
European Physical Journal D -- Atoms, Molecules, Clusters & Optical Physics
Springer Science & Business Media B.V.
51
1
89-96
Yes (verified by ORBi)
International
1434-6060
New York
NY
[en] Nucleic acids
[en] Collision induced dissociation in mass spectrometry of protonated nucleosides typically yields as charged fragment the protonated nucleobase augmented by one hydrogen transferred from the 2'-deoxyribose, [ bH(2)]. The origin of the hydrogen transfer for the protonated 2'-deoxyguanosine has been investigated by collision induced dissociation of deuterium enriched molecules. [ 1'-H-2] 2'-deoxyguanosine, [ 2',2aEuro(3)-H-2(2)] 2'-deoxyguanosine, and [ 5',5aEuro(3)-H-2(2)] 2'-deoxyguanosine have been studied and deuterium/hydrogen back exchange has been used following complete or partial exchange of the 2'-deoxyguanosine in solution. Based on the fragmentation of the deuterated molecular ions, the hydrogen transferred from the 2'-deoxyribose to the guanine is found to originate from the O5' atom on the 2'-deoxyribose. A fragmentation mechanism involving a hydrogen shift from the 2' position to the oxygen atom O5' and supported by the kinetic isotope effect on the survival yield curves is proposed. The influence of the 2'-deoxyguanosine conformation on the hydrogen transfer and the fragmentation mechanism is discussed.
Giga-Systems Biology and Chemical Biology
University of Liege ; Walloon Region
Researchers
http://hdl.handle.net/2268/13021
10.1140/epjd/e2008-00149-4
http://springerlink.metapress.com/content/x0r4283162017372/?p=2365f1eee36b4c729956f1d4658c0fa3&pi=10

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Restricted access
Eur Phys J D2009Greisch.pdfPublisher postprint297.97 kBRequest copy

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.