Reference : Cloning and expression analysis of an inducible HSP70 gene from tilapia fish
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/13017
Cloning and expression analysis of an inducible HSP70 gene from tilapia fish
English
Molina, Alfredo [> > > >]
Biemar, Frédéric [> > > >]
Muller, Ferenc [University of Southampton > > > >]
Iyengar, Arati [University of Southampton > > > >]
Prunet, Patrick [University of Southampton > > > >]
Maclean, Norman [University of Southampton > > > > > >]
Martial, Joseph mailto [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
Muller, Marc mailto [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
2000
FEBS Letters
Elsevier Science
474
1
5-10
Yes (verified by ORBi)
International
0014-5793
Amsterdam
The Netherlands
[en] Amino Acid Sequence ; Animals ; Base Sequence ; Carps/metabolism ; *Cloning, Molecular ; DNA/chemistry ; *Gene Expression ; HSP70 Heat-Shock Proteins/chemistry/*genetics ; Hot Temperature ; Molecular Sequence Data ; Promoter Regions, Genetic ; RNA, Messenger/metabolism ; Regulatory Sequences, Nucleic Acid ; Reverse Transcriptase Polymerase Chain Reaction ; TATA Box ; Tilapia/*genetics ; Transfection ; Zebrafish/embryology
[en] We isolated and characterized the tilapia (Oreochromis mossambicus) HSP70 gene, highly homologous to other HSP70 genes. A dramatic increase of tilapia HSP70 mRNA levels was observed after heat shock of whole animals in all organs tested. Reporter constructs were tested for transient expression in carp cells and in microinjected zebrafish embryos. The entire isolated regulatory region (-851/+157) was able to mediate heat shock inducible expression of the reporter gene, with no preference for a particular tissue. Our studies represent the first transcriptional analysis of a HSP70 promoter from fish, revealing a powerful tool to direct controlled, tissue-independent gene expression in fish.
http://hdl.handle.net/2268/13017
10.1016/S0014-5793(00)01538-6
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T36-40B1WS3-2&_user=532038&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000026659&_version=1&_urlVersion=0&_userid=532038&md5=c63d83f731968e344c667a50628c12f5
2000/06/01

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