Reference : Gene structure and promoter function of a teleost ribosomal protein: a tilapia (Oreochro...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/13014
Gene structure and promoter function of a teleost ribosomal protein: a tilapia (Oreochromis mossambicus) L18 gene
English
Molina, Alfredo [> > > >]
Iyengar, Arati [University of Southampton > > > >]
Marins, Luis F. [University of Southampton > > > >]
Biemar, Frédéric [> > > >]
Hanley, Sean [National University of Ireland > > > >]
Maclean, Norman [University of Southampton > > > >]
Smith, Terry J. [National University of Ireland > > > >]
Martial, Joseph mailto [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
Muller, Marc mailto [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
2001
Biochimica et Biophysica Acta
1520
3
195-202
Yes (verified by ORBi)
International
0006-3002
[en] Amino Acid Sequence ; Animals ; Base Sequence ; Cell Line ; Cloning, Molecular ; Embryo, Nonmammalian ; Genes, Reporter ; Microinjections ; Molecular Sequence Data ; *Promoter Regions, Genetic ; Reverse Transcriptase Polymerase Chain Reaction ; Ribosomal Proteins/chemistry/*genetics ; Sequence Alignment ; Tilapia/embryology/*genetics ; Zebrafish
[en] We have cloned and characterized a tilapia (Oreochromis mossambicus) L18 ribosomal protein gene, including the complete transcribed region and 488 bp of upstream regulatory sequences. We have also isolated two L18 cDNAs from another tilapia (Oreochromis niloticus) with a few conservative nucleotide differences. Our results suggest the presence of two genes in both species. Reporter constructs were tested for transient expression in CV1 cells and in microinjected zebrafish and tilapia embryos. The tilapia L18 promoter was able to drive expression of the reporter gene in all three experiments, with no apparent preference for a particular tissue. The tilapia L18 promoter is therefore likely to be a powerful tool to drive tissue-independent gene expression in fish.
Applied and Fundamental FISH Research Center - AFFISH-RC ; Giga-Development and Stem Cells
http://hdl.handle.net/2268/13014
10.1016/S0167-4781(01)00272-X
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T1V-441373D-2&_user=532038&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000026659&_version=1&_urlVersion=0&_userid=532038&md5=9019ba1bd509be0ca243e689c328e86b
2001/09/22

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