Reference : Effect of Additives on Freeze-Drying and Storage of Yarrowia lipolytica Lipase.
Scientific journals : Article
Life sciences : Microbiology
Life sciences : Biotechnology
http://hdl.handle.net/2268/130097
Effect of Additives on Freeze-Drying and Storage of Yarrowia lipolytica Lipase.
English
Darvishi, Farshad [> >]
Destain, Jacqueline mailto [Université de Liège - ULg > Chimie et bio-industries > Bio-industries >]
Nahvi, Iraj [> >]
Thonart, Philippe mailto [Université de Liège - ULg > Département des sciences de la vie > Biochimie et microbiologie industrielles >]
Zarkesh-Esfahani, Hamid [> >]
2012
Applied Biochemistry and Biotechnology
Yes (verified by ORBi)
International
0273-2289
[en] The extracellular lipase of Yarrowia lipolytica presents numerous potentialities for biotechnological applications. This work describes the development and storage of powders obtained from supernatants containing Y. lipolytica lipase by freeze-drying as downstream process that is important in obtaining a stable lipase powder with high enzymatic activity. Lipase was produced by Y. lipolytica U6 mutant strain in 20-L bioreactor. Non-concentrated cell-free culture supernatant samples were supplemented with different concentrations (0.5-1 %) of maltodextrin and glycerol as additives to freeze-drying. Effects of additives, temperature, pH, and storage time on lipase powders were determined. After addition of additives, freeze-drying yield increased 3.5-fold compared to supernatant without additive. Maltodextrin with 0.5 % concentration gave the best protection of lipase during dehydration treatment and its freeze-drying yield (77 %) is better than other formulations. Lipase powders were stored at 4 and 25 degrees C for 46 weeks without loss of lipase activity. A common impediment to the production of commercial enzyme is their low-stability aqueous solutions. The present study shows that freeze-dried lipase powders of Y. lipolytica have good stability for storage and various applications.
http://hdl.handle.net/2268/130097
10.1007/s12010-012-9844-z

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