Reference : Anomalous behaviour in the diffusion of polyethylene oxide through dialysis membrane
Scientific congresses and symposiums : Poster
Social & behavioral sciences, psychology : Neurosciences & behavior
http://hdl.handle.net/2268/130084
Anomalous behaviour in the diffusion of polyethylene oxide through dialysis membrane
English
Vignisse, Julie mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Biochimie et physiologie humaine et pathologique >]
Gustin, Audrey []
Lespineux [Université de Liège - ULg > Centre interfacultaire des biomatériaux > > >]
Sevrin, Chantal mailto [Université de Liège - ULg > > Centre interfacultaire des biomatériaux (CEIB) >]
Grandfils, Christian mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Biochimie et physiologie générales, et biochimie humaine >]
1-Apr-2009
A0
No
No
International
Biomedica 2009
du 1er au 2 avril 2009
Liège
Belgique
[en] Dialysis is a common technique adopted in biochemistry to purify biopharmaceutical drugs. This methodology is also of interest in macromolecular chemistry and pharmaceutical nanotechnology in order to purify synthetic macromolecules and nanodrug carriers designed for drug delivery purposes. However, based on their original applications, the diffusion characteristic of the dialysis membrane is given in respect to the diffusion rate of globular proteins. So the diffusion capacity is function of molecular weight cut-off, i.e. corresponding to the maximum molecular weight of a globular macromolecule to be able to cross the membrane.

The diffusion kinetics of synthetic macromolecules is expected to differ significantly from globular proteins due to at least the following differences :
Specific relationship between hydrodynamic diameter and molecular weight,
Flexibility
Ionic density
Solubility/miscibility/adsorption behaviour with the dialysis membrane
Polymer chain entanglement above a critical concentration.

In view to validate the application of this technique to purify synthetic macromolecules, we have compared the diffusion ability of neutral polyethyetylene oxide (PEO) standards or poly(dimethyl-aminoethyl-methacrylate) (PMADAM) to protein standards (human insulin and ovalbumin).
http://hdl.handle.net/2268/130084

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