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Regulation of Histamine Release from Human Bronchoalveolar Lavage Mast Cells by Stem Cell Factor in Several Respiratory Diseases
Louis, Renaud; Tilkin, P.; Poncelet, M. et al.
1995In Allergy, 50 (4), p. 340-8
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Abstract :
[en] We investigated the effects of stem cell factor (SCF) on histamine release (HR) from human bronchoalveolar lavage (BAL) mast cells. BAL cells were recovered from lavage performed in patients undergoing clinical bronchoscopy. SCF (0.02-20 ng/ml), which is by itself a poor secretagogue (mean +/- SEM HR: 3.7 +/- 0.9%; n = 27), strongly enhanced HR induced by anti-IgE in a concentration-related manner. Significant potentiation began at 0.2 ng/ml (30 +/- 10%; p < 0.05; n = 12) and reached a plateau at 2 ng/ml (40 +/- 10%; P < 0.01 at 2 ng/ml and 45 +/- 10%; P < 0.01 at 20 ng/ml; n = 12). In contrast, SCF failed to enhance HR induced by calcium ionophore A23187. Among the BAL cell samples initially unresponsive to anti-IgE (55% of samples), 36% (10/28) were converted to responders if the cells were shortly preincubated with SCF. In 25% of samples (7/27), SCF (20 ng/ml) caused direct HR of 10 +/- 2.1%. The mast cells which released histamine when challenged with SCF also secreted higher levels of histamine in response to anti-IgE and calcium ionophore than those nonresponsive to SCF. While interleukin (IL)-3 and IL-5 (20 ng/ml) were unable to modulate immunologic HR, GM-CSF (20 ng/ml) produced significant potentiation (P < 0.05), which was, however, smaller than that observed with SCF.(ABSTRACT TRUNCATED AT 250 WORDS)
Disciplines :
Cardiovascular & respiratory systems
Author, co-author :
Louis, Renaud ;  Université de Liège - ULiège > Département des sciences cliniques > Pneumologie - Allergologie
Tilkin, P.
Poncelet, M.;  Université de Liège - ULiège > Pneumologie-Allergologie
Corhay, Jean-Louis ;  Université de Liège - ULiège > Pneumologie-Allergologie
Mendez, P.
Weber, T.
Radermecker, Maurice ;  Université de Liège - ULiège > Services généraux (Faculté de médecine) > Relations académiques et scientifiques (Médecine)
Language :
English
Title :
Regulation of Histamine Release from Human Bronchoalveolar Lavage Mast Cells by Stem Cell Factor in Several Respiratory Diseases
Publication date :
April 1995
Journal title :
Allergy
ISSN :
0105-4538
eISSN :
1398-9995
Publisher :
Wiley-Blackwell, Oxford, United Kingdom
Volume :
50
Issue :
4
Pages :
340-8
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 30 November 2009

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