Reference : Reproductive impact and proteomic analysis of androgenic and anti-androgenic disrupto...
Scientific congresses and symposiums : Poster
Life sciences : Environmental sciences & ecology
http://hdl.handle.net/2268/129257
Reproductive impact and proteomic analysis of androgenic and anti-androgenic disruptors on the hermaphroditic freshwater gastropod Lymnaea stagnalis
English
Giusti, Arnaud mailto [Université de Liège - ULg > Département de Biologie, Ecologie et Evolution > Ecologie animale et écotoxicologie >]
Ducrot, Virginie [Institut Scientifique de Recherche Agronomique - INRA > UMR985 > Equipe Ecotoxicologie et santé des milieux aquatiques > >]
Joaquim-Justo, Célia mailto [Université de Liège - ULg > Département de Biologie, Ecologie et Evolution > Ecologie animale et écotoxicologie >]
Lagadic, Laurent [Institut Scientifique de Recherche Agronomique - INRA > UMR985 > Equipe Ecotoxicologie et santé des milieux aquatiques > >]
Leprince, Pierre mailto [Université de Liège - ULg > > GIGA - Neurosciences >]
De Pauw, Edwin mailto [Université de Liège - ULg > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.) >]
Thomé, Jean-Pierre mailto [Université de Liège - ULg > Département de Biologie, Ecologie et Evolution > Ecologie animale et écotoxicologie >]
Nov-2011
No
No
International
32nd SETAC North America annual meeting
du 13 au 17 Novembre 2012
SETAC
Boston
USA
[en] Endocrine disruption ; Gastropods ; Proteomic
[en] In this study, e ects of two androgens (tributyltin and testosterone),
one antiandrogen (cyproterone acetate) and one estrogen (chlordecone) on
growth and reproduction were investigated in the hermaphrodite gastropod
Lymnaea stagnalis. In this study, exposure to a range of concentrations (ng/l
to μg/l) of each chemical was performed during 21 days. e number of
clutches and the number of eggs per clutch were monitored. A decrease in
clutches laid per snail was observed after exposure to tributyltin (540, 1180
and 2600 ng/l) and chlordecone (10, 22, 50 and 110 μg/l). A signi cant
decrease in egg laid per snail was observed after exposure to tributyltin (540,
1180 and 2600 ng/l) and chlordecone (50 and 110 μg/l). An increase of egg
abnormalities ratio in exposed snails (atrophied albumen, polyembryonicity,...)
was observed in L. stagnalis following exposure to testosterone (2, 22,
50 and 110 ng/l), cyproterone acetate (4,5 and 50 μg/l), tributyltin (110
and 244 ng/l) and chlordecone (4,5 and 10 μg/l). Investigation of alteration
in protein expression in exposed snails was performed using proteomic
analysis such as 2D-DIGE. Mass spectrometry identi cation was performed
on proteins with altered expression. We could establish correlation between
reproductive endpoints and changes in proteins involved in egg formation
and in egg laying were underlined. Egg yolk ferritin, the main protein of egg
yolk, was shown to be reduced signi cantly in relationship with a decrease
of egg yolk quality after exposure to tributyltin 540 ng/l and cyproterone
acetate 4,5 μg/l. Ovipostatin, a protein proved to reduce egg masses, was
signi cantly over expressed in snails exposed to 50 μg/l of chlordecone and
were in relationship with a reduction of clutches laid by individuals. Further
western blot analysis on those proteins involved in the reproduction are
underway. ese analysis will enable us to con rm and re ne with more
speci city the 2D-DIGE results for the selected proteins. e results of this
study can help to establish new biomarkers of exposure of endocrine disruptors
in freshwater environment and can provide new insight on mode of
action of endocrine disruptors in L. stagnalis.
Centre Interfacultaire d'Analyse des Résidus en Traces - CART ; INRA
Fonds pour la formation à la Recherche dans l'Industrie et dans l'Agriculture (Communauté française de Belgique) - FRIA
http://hdl.handle.net/2268/129257

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