ORBi: Giusti Arnaud - Reproductive impact and proteomic analysis of androgenic and anti-androgenic disruptors on the hermaphroditic freshwater gastropod Lymnaea stagnalis

Reference : Reproductive impact and proteomic analysis of androgenic and anti-androgenic disruptors ...


	Document type :	Scientific congresses and symposiums : Poster
	Discipline(s) :	Life sciences : Environmental sciences & ecology
	To cite this reference:	http://hdl.handle.net/2268/129257

Title :	Reproductive impact and proteomic analysis of androgenic and anti-androgenic disruptors on the hermaphroditic freshwater gastropod Lymnaea stagnalis
Language :	English
Author, co-author :	Giusti, Arnaud [Université de Liège - ULg > Département de Biologie, Ecologie et Evolution > Ecologie animale et écotoxicologie >]
	Ducrot, Virginie [Institut Scientifique de Recherche Agronomique - INRA > UMR985 > Equipe Ecotoxicologie et santé des milieux aquatiques > >]
	Joaquim-Justo, Célia [Université de Liège - ULg > Département de Biologie, Ecologie et Evolution > Ecologie animale et écotoxicologie >]
	Lagadic, Laurent [Institut Scientifique de Recherche Agronomique - INRA > UMR985 > Equipe Ecotoxicologie et santé des milieux aquatiques > >]
	Leprince, Pierre [Université de Liège - ULg > > GIGA - Neurosciences >]
	De Pauw, Edwin [Université de Liège - ULg > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.) >]
	Thomé, Jean-Pierre [Université de Liège - ULg > Département de Biologie, Ecologie et Evolution > Ecologie animale et écotoxicologie >]
Publication date :	Nov-2011
Peer reviewed :	No
On invitation :	No
Audience :	International
Event name :	32nd SETAC North America annual meeting
Event date :	du 13 au 17 Novembre 2012
Event organizer :	SETAC
Event place (city) :	Boston
Event country :	USA
Keywords :	[en] Endocrine disruption ; Gastropods ; Proteomic
Abstract :	[en] In this study, e ects of two androgens (tributyltin and testosterone), one antiandrogen (cyproterone acetate) and one estrogen (chlordecone) on growth and reproduction were investigated in the hermaphrodite gastropod Lymnaea stagnalis. In this study, exposure to a range of concentrations (ng/l to μg/l) of each chemical was performed during 21 days. e number of clutches and the number of eggs per clutch were monitored. A decrease in clutches laid per snail was observed after exposure to tributyltin (540, 1180 and 2600 ng/l) and chlordecone (10, 22, 50 and 110 μg/l). A signi cant decrease in egg laid per snail was observed after exposure to tributyltin (540, 1180 and 2600 ng/l) and chlordecone (50 and 110 μg/l). An increase of egg abnormalities ratio in exposed snails (atrophied albumen, polyembryonicity,...) was observed in L. stagnalis following exposure to testosterone (2, 22, 50 and 110 ng/l), cyproterone acetate (4,5 and 50 μg/l), tributyltin (110 and 244 ng/l) and chlordecone (4,5 and 10 μg/l). Investigation of alteration in protein expression in exposed snails was performed using proteomic analysis such as 2D-DIGE. Mass spectrometry identi cation was performed on proteins with altered expression. We could establish correlation between reproductive endpoints and changes in proteins involved in egg formation and in egg laying were underlined. Egg yolk ferritin, the main protein of egg yolk, was shown to be reduced signi cantly in relationship with a decrease of egg yolk quality after exposure to tributyltin 540 ng/l and cyproterone acetate 4,5 μg/l. Ovipostatin, a protein proved to reduce egg masses, was signi cantly over expressed in snails exposed to 50 μg/l of chlordecone and were in relationship with a reduction of clutches laid by individuals. Further western blot analysis on those proteins involved in the reproduction are underway. ese analysis will enable us to con rm and re ne with more speci city the 2D-DIGE results for the selected proteins. e results of this study can help to establish new biomarkers of exposure of endocrine disruptors in freshwater environment and can provide new insight on mode of action of endocrine disruptors in L. stagnalis.
Research units :	Centre Interfacultaire d'Analyse des Résidus en Traces - CART ; INRA
Funders :	Fonds pour la formation à la Recherche dans l'Industrie et dans l'Agriculture (Communauté française de Belgique) - FRIA
Permalink :	http://hdl.handle.net/2268/129257

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