Reference : Identification and Relative-quantification of Glycans by Matrix-assisted Laser Desorp...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/128670
Identification and Relative-quantification of Glycans by Matrix-assisted Laser Desorption/Ionization In-Source Decay with Hydrogen Abstraction
English
Akasawa, Daiki mailto [Université de Liège - ULg > Département de Chimie (Sciences) > GIGA-R: Laboratoire de spectrométrie de masse (LSM) > >]
Smargiasso, Nicolas mailto [Université de Liège - ULg > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.) >]
De Pauw, Edwin mailto [Université de Liège - ULg > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.) >]
6-Aug-2012
Analytical Chemistry
American Chemical Society
just accepted paper
Yes (verified by ORBi)
International
0003-2700
1520-6882
Washington
DC
[en] Glycans ; MALDI-ISD ; Mass Spectrometry
[en] The use of specific matrices allows enhancing the scope of in-source decay (ISD) applications
in matrix-assisted laser desorption ionization (MALDI) thanks to the specificity of
analyte-matrix chemistry. The use of an oxidizing matrix, 5-nitrosalicylic acid (5-NSA) for
MALDI-ISD of glycans is shown to promote fragmentation pathways involving radical
precursors. Both glycosidic and cross-ring cleavages are promoted by hydrogen abstraction
from hydroxyl group of glycans by 5-NSA molecules. Cross-ring cleavage ions are potentially
useful in linkage analysis, one of the most critical steps of glycan characterization. Moreover,
we show here that isobaric glycans could be distinguished by structure specific ISD ions, and
that the molar ratio of glycan isomers in the mixture can be estimated from their fragment
ions abundance. The use of 5-NSA also opens the possibility to perform pseudo-MS3 analysis
of glycans. Therefore, MALDI-ISD with 5-NSA is a useful method for identification of
glycans and semi-quantitative analysis of mixture of glycan isomers.
GIGA-R ; Departement de Chimie ; Mass Spectrometry Laboratory
Japan Society for the Promotion of Science for Young Scientists
http://hdl.handle.net/2268/128670
10.1021/ac3014737
https://vpn.gw.ulg.ac.be/doi/abs/10.1021/,DanaInfo=pubs.acs.org+ac3014737

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