|Reference : Factors of Learning in Mouse Models of Memory and Evaluation of Effects of Dimebon|
|Dissertations and theses : Master's dissertation|
|Social & behavioral sciences, psychology : Neurosciences & behavior|
|Factors of Learning in Mouse Models of Memory and Evaluation of Effects of Dimebon|
|[fr] Facteurs d'apprentissage chez les Modèles Murins de Mémoire et Evaluation des Effets du Dimebon|
|Vignisse, Julie [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Biochimie et physiologie humaine et pathologique >]|
|Université de Liège|
|Master en Sciences Biomédicales à finalité approfondie (Neuroscience)|
|[en] Dimebon ; Cognition ; Mice ; Factors of learning ; age ; stress ; strain|
|[en] 1. Factors of learning in mouse models of memory
The impact of several most relevant biological factors in contextual learning in mice was investigated in the first part of our study. It is of general importance to estimate an impact of each of them in animals’ acquisition of various learning tasks during practical experimental work. First, factors of strain differences, aging and stress, investigated in our work can confound behavioural testing in memory paradigms. Second, they can be used as a basis of behavioural models of memory deficits and, thus, model pathological conditions in humans.
Step-down avoidance task performed on three strains (namely C57BL/6N, CD1 and BALB/c) has clearly highlighted differences of abilities in learn contextual memory in these strains. The step-down avoidance task was also applied on C57BL/6N old of 3-months or 7-months; older mice displayed lower scores of memory then the young onces, thus, even mild aging impaired contextual learning in this strain. Third, we investigated learning of C57BL/6 mice subjected to chronic stress in a fear conditioning paradigm. Our study showed that the acquisition of this task was disrupted in stressed group, as reflected by decreased scores of freezing behavior. The assessment of investigated here factors of learning provides a possibility to validate animal models of memory and evaluate their sensitivity.
2. Evaluation of effects of dimebon
Dimebon, a heterocyclic compounds previously adopted in clinic as antihistaminic, has recently revealed enhancing cognitive properties in pre-clinical and clinical studies The aim of the present study was to identify the most optimal dosing and adequate memory test(s), which would be sensitive to the memory enhancing effects of dimebon. Amongst the different studies realized, Dimebon revealed enhancement in memory in the step-down avoidance test, a one trial hippocampus-dependant task, at dose 0.5 mg/kg administered acutely and in the Y-Maze test, a multiple training paradigm at dose 0.1 mg/kg delivered chronically. In conclusion, our study supports therefore that the step-down avoidance and Y-maze paradigms are the most convenient assays allowing a rapid and reliable assessment of effects of of drugs with memory enhancing properties such as dimebon and dimebon-like.
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