Reference : DERP6 (ELP5) and C3ORF75 (ELP6) regulate tumorigenicity and migration of melanoma cel...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/128331
DERP6 (ELP5) and C3ORF75 (ELP6) regulate tumorigenicity and migration of melanoma cells as subunits of Elongator
English
Close, Pierre mailto [Université de Liège - ULg > Département de pharmacie > Chimie médicale >]
Gillard, Magali mailto []
Ladang, Aurélie mailto [Université de Liège - ULg > > > GIGA - Membres]
Jiang, Zheshen mailto [Université de Liège - ULg > > > GIGA - Membres]
Papuga, Jessica mailto [Université de Liège - ULg > Département de pharmacie > Chimie médicale >]
Hawkes, Nicola []
Nguyen, Laurent mailto [Université de Liège - ULg > Département des sciences cliniques > Neurologie >]
CHAPELLE, Jean-Paul mailto [Centre Hospitalier Universitaire de Liège - CHU > > Chimie médicale >]
Bouillenne, Fabrice mailto [Université de Liège - ULg > > Centre d'ingénierie des protéines >]
Svejstrup, Jesper []
Fillet, Marianne mailto [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
Chariot, Alain mailto [Université de Liège - ULg > Département de pharmacie > Chimie médicale >]
1-Aug-2012
Journal of Biological Chemistry
American Society for Biochemistry and Molecular Biology
Yes (verified by ORBi)
International
0021-9258
1083-351X
Baltimore
MD
[en] migration ; melanoma ; tumorigenicity
[en] The Elongator complex is composed of
6 subunits (Elp1-Elp6) and promotes RNAPII
transcript elongation through histone
acetylation in the nucleus as well as tRNA
modification in the cytoplasm. This
acetyltransferase complex directly or
indirectly regulates numerous biological
processes ranging from exocytosis and
resistance to heat shock in yeast to cell
migration and neuronal differentiation in
higher eukaryotes. The identity of human
ELP1 through ELP4 has been reported but
human ELP5 and ELP6 have remained
uncharacterized. Here, we report that DERP6
(ELP5) and C3ORF75 (ELP6) encode these
subunits of human Elongator. We further
investigated the importance and function of
these two subunits by a combination of
biochemical analysis and cellular assays. Our
results show that DERP6/ELP5 is required
for the integrity of Elongator and directly
connects ELP3 to ELP4. Importantly, the
migration and tumorigenicity of melanomaderived
cells are significantly decreased upon
Elongator depletion through ELP1 or ELP3.
Strikingly, DERP6/ELP5 and
C3ORF75/ELP6-depleted melanoma cells
have similar defects, further supporting the
idea that DERP6/ELP5 and C3ORF75/ELP6
are essential for Elongator function. Together,
our data identify DERP6/ELP5 and
C3ORF75/ELP6 as key players for migration,
invasion and tumorigenicity of melanoma
cells, as integral subunits of Elongator.
Giga-Signal Transduction
http://hdl.handle.net/2268/128331
10.1074/jbc.M112.402727
http://www.jbc.org/content/early/2012/08/01/jbc.M112.402727

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