Reference : Optimization of biosurfactant lipopeptide production from Bacillus subtilis S499 by Plac...
Scientific journals : Article
Life sciences : Agriculture & agronomy
http://hdl.handle.net/2268/12803
Optimization of biosurfactant lipopeptide production from Bacillus subtilis S499 by Plackett-Burman design
English
Jacques, P. [> > > >]
Hbid, C. [> > > >]
Destain, Jacqueline mailto [Université de Liège - ULg > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech >]
Razafindralambo, Hary [Université de Liège - ULg > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech >]
Paquot, Michel mailto [Université de Liège - ULg > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech >]
De Pauw, Edwin mailto [Université de Liège - ULg > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.) >]
Thonart, Philippe mailto [Université de Liège - ULg > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech - Biochimie et microbiologie industrielles >]
1999
Applied Biochemistry and Biotechnology
Humana Press
77-9
223-233
Yes (verified by ORBi)
0273-2289
Totowa
NJ
[en] Bacillus subtilis S499 is well-known for its ability to produce two families of surfactant lipopeptides: Iturin A and Surfactin S1. Fermentation optimization for this strain was performed to amplify the surfactant production. Ten active variables were analyzed by two successive Plackett-Burman designs, consisting respectively of 12 and 16 experiments to give an optimized medium. The amount of biosurfactant lipopeptides in the supernatant of a culture carried out in this optimized medium was about five times higher than that obtained in nonoptimized rich medium. The analysis of the surfactant molecules produced in such optimized conditions has revealed the presence of a third family of lipopeptides: the fengycins. The time-dependent production of these three families of molecules in bioreactors showed that surfactin S1 is produced during the exponential phase and iturin A and fengycins during the stationary phase.
http://hdl.handle.net/2268/12803
also: http://hdl.handle.net/2268/60709

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