ORBi: Jaïdane Hela - Persistent infection of thymic epithelial cells with coxsackievirus B4 results in a decreased expression of insulin-like growth factor 2

Reference : Persistent infection of thymic epithelial cells with coxsackievirus B4 results in a decr...


	Document type :	Scientific journals : Article
	Discipline(s) :	Human health sciences : Immunology & infectious disease Human health sciences : Endocrinology, metabolism & nutrition
	To cite this reference:	http://hdl.handle.net/2268/128026

Title :	Persistent infection of thymic epithelial cells with coxsackievirus B4 results in a decreased expression of insulin-like growth factor 2
Language :	English
Author, co-author :	Jaïdane, Hela [Université Lille 2 - CHRU > Laboratoire de Virologie EA3610 > > >]
	Caloone, Delphine [Université Lille 2 - CHRU > Laboratoire de Virologie EA3610 > > >]
	Lobert, Pierre-Emmanuel [Université de Lille 2-CHRU > Laboratoire de Virologie EA3610 > > >]
	Sane, Famara [Université de Lille 2-CHRU > Laboratoire de Virologie EA3610 > > >]
	Dardenne, Olivier [Université de Liège - ULg > GIGA Research > Centre d'Immunologie > >]
	Naquet, Philippe [Centre d'Immunologie Marseille-Luminy/INSERM-CNRS > > > >]
	Gharbi, Jawar [Université de Monastir - Faculté de Pharmacie > Laboratoire des Maladies Transmissibles > > >]
	Geenen, Vincent [Université de Liège - ULg > > Centre d'immunologie >]
	Hober, Didier [Université de Lille 2-CHRU > Laboratoire de Virologie EA3610 > > >]
Publication date :	2012
Journal title :	Journal of Virology
Publisher :	American Society for Microbiology (ASM)
Volume :	86
Pages :	11151-11162
Peer reviewed :	Yes (verified by ORBi)
Audience :	International
ISSN :	0022-538X
e-ISSN :	1098-5514
City :	Washington
Country :	DC
Keywords :	[en] Thymus ; Coxsackievirus ; IGF-2
Abstract :	[en] It has been hypothesized that a disturbance of central self-tolerance to islet β-cell may play a role in the enteroviral pathogenesis of type 1 diabetes. Whether enteroviruses can induce an impaired expression of β-cell self-antigens in thymic epithelial cells has been investigated in a murine thymic epithelial (MTE) cell line. This cell line was permissive to the diabetogenic strain CV-B4 E2 and spontaneously expressed type 2 insulin-like growth factor (Igf2), the dominant self-antigen of the insulin family. In this model, a persistent replication of CV-B4 E2 was obtained as attested by the prolonged detection of intracellular positive and negative-strand viral RNA by RT-PCR, and capsid protein VP1 by IF and by the release of infectious particles in culture supernatant fluids. The chronic stage of the infection was characterized by a low proportion of VP1-positive cells (1-2%) whereas many cells harbored enteroviral RNA as displayed by RT-PCR without extraction applied directly on a few cells. Igf2 mRNA and IGF-2 protein were dramatically decreased in CV-B4 E2-infected MTE cultures compared with mock-infected cultures, whereas housekeeping and Il6 genes expression were maintained and Igf1 mRNA was decreased but at a lower extent. Inoculation of CV-B3-, CV-B4 JVB- or Echovirus 1 resulted in a low level of IGF-2 in culture supernatant fluids as well, whereas HSV-1 stimulated the production of the protein. Thus, a persistent infection of a thymic epithelial cell line with enteroviruses, like CV-B4 E2 can result in a disturbed production of IGF-2, a protein involved in central self-tolerance towards islet β-cells.
Funders :	Commission européenne : Direction générale de la Recherche
Name of the research project :	FP6 Integrated Project Eurothymaide
Target :	Researchers ; Professionals
Permalink :	http://hdl.handle.net/2268/128026
Other URL :	http://reflexions.ulg.ac.be/diabete

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