Article (Scientific journals)
High salt-tolerant protease from a potential biocontrol agent bacillus pumilus M3-16
Essghaier, Badiaa; Bajji, Mohammed; Jijakli, Haissam et al.
2009In Annals of Microbiology, 59 (3), p. 553-558
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Keywords :
Bacillus pumilus; halotolerant; antifungal protease; biocontrol agent
Abstract :
[en] In this paper, we investigate the characterization and evaluation of the antifungal protease activity from a halotolerant strain M3-16 of Bacillus pumilus, earlier isolated from a shallow salt lake in Tunisia. Protease enzyme was highly induced by the pathogen tested in vitro (27.4 U/ml). This is the first report on high salt-tolerant protease from B. pumilus, since it was active at high salinity (from 5 to 30% NaCl, w/v) as well as in the absence of salinity. This enzyme showed optimal activity at 60 °C and pH 8. At 80 °C and 30 min, the enzyme retained up to 91% and it showed stability over a wide pH range (from pH 5 to 11). The enzyme was found to be monomer with an estimated molecular mass of 31 kDa. The amino acid sequence showed high similarity (94%) to ATP-dependent protease from B. pumilus strain ATCC 7061. Thus, our alkaline thermostable and high salt-tolerant protease induced by a phytopathogenic fungus, could be useful for application in diverse areas such as biotechnology alimentary and agronomy industries.
Disciplines :
Phytobiology (plant sciences, forestry, mycology...)
Author, co-author :
Essghaier, Badiaa
Bajji, Mohammed
Jijakli, Haissam  ;  Université de Liège - ULiège > Sciences agronomiques > Phytopathologie
Boudabous, Abdellatif
Sadfi-Zouaoui, Najla
Language :
English
Title :
High salt-tolerant protease from a potential biocontrol agent bacillus pumilus M3-16
Publication date :
2009
Journal title :
Annals of Microbiology
ISSN :
1590-4261
eISSN :
1869-2044
Publisher :
Springer
Volume :
59
Issue :
3
Pages :
553-558
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 19 July 2012

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