Reference : Optimization of the liquid chromatography enantioseparation of chiral acidic compounds u...
Scientific journals : Article
Human health sciences : Pharmacy, pharmacology & toxicology
http://hdl.handle.net/2268/127588
Optimization of the liquid chromatography enantioseparation of chiral acidic compounds using cellulose tris(3-chloro-4-methylphenylcarbamate) as chiral selector and polar organic mobile phases.
English
Dossou, K. S. S. [> >]
Farcas, Elena mailto [Université de Liège - ULg > > > >]
Servais, Anne-Catherine mailto [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
CHIAP, Patrice mailto [Centre Hospitalier Universitaire de Liège - CHU > > Pharmacologie clinique >]
Chankvetadze, B. [> >]
Crommen, Jacques mailto [Université de Liège - ULg > Département de pharmacie > Département de pharmacie >]
Fillet, Marianne mailto [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
2012
Journal of Chromatography. A
1234
56-63
Yes (verified by ORBi)
International
0021-9673
1873-3778
Netherlands
[en] Liquid chromatography ; Enantioseparation ; Chiral acidic compounds ; Chlorinated cellulose based chiral stationary phases ; Polar organic mobile phases ; Optimization
[en] The LC enantioseparation of chiral acidic and zwitterionic drugs selected as model compounds was optimized using chlorine containing cellulose based chiral stationary phases and polar organic mobile phases. The main solvent of the mobile phase was acetonitrile, the temperature was settled at 25 degrees C and a stationary phase with cellulose tris(3-chloro-4-methylphenylcarbamate) as chiral selector (3-Cl-4-Me-PC) was selected. In the screening step, the nature and concentration of both acidic and basic additives were found to have a significant effect on retention, selectivity and resolution. Acetic acid (AcA) was selected as acidic additive for the optimization step since it could lead to the enantioseparation of more acidic compounds than trifluoroacetic acid (TFA) and formic acid (FA), while among the three basic additives tested, diethylamine (DEA) most often gave better results with respect to enantioresolution and selectivity than butylamine (BuA) and triethylamine (TEA). The optimization was performed using a central composite face-centered design with two factors, namely the concentration of acetic acid (0.1-0.3%) and the concentration of DEA (0.01-0.1%) in the mobile phase. On the basis of the results obtained in the screening and optimization steps, a strategy for the rapid development of methods for the enantioseparation of acidic or neutral compounds was proposed.
CIRM
http://hdl.handle.net/2268/127588
Copyright A(c) 2012 Elsevier B.V. All rights reserved.

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