Type Iv Collagen Induces Matrix Metalloproteinase 2 Activation in Ht1080 Fibrosarcoma Cells

[en] Matrix metalloproteinase 2 (MMP-2) activation has been described as a "master switch" which triggers tumor spread and metastatic progression. We show here that type IV collagen, a major component of basement membranes, promotes MMP-2 activation by HT1080 cells. When plated on plastic, HT1080 cells constitutively processed the 66-kDa pro-MMP-2 into a 62-kDa intermediate activated form, most probably through a membrane type (MT) 1 MMP-dependent mechanism. In the presence of type IV collagen, part of this intermediate form was further processed to fully activated 59-kDa MMP-2. This activation was prevented by tissue inhibitor of MMP (TIMP)-2 and a broad-spectrum hydroxamic acid-based synthetic MMP inhibitor (GI129471). Type IV collagen-mediated pro-MMP-2 activation did not involve either a transcriptional modulation of MMP-2, MT1-MMP, or TIMP-2 expression nor any alteration of MT1-MMP protein synthesis or processing. An inverse relationship between MMP-2 activation and the concentration of secreted TIMP-2 was observed. This is consistent with our previous report that TIMP-2 degradation is probably linked to the MT1-MMP-dependent MMP-2 activation mechanism. Because invasive tumor cells must breach basement membranes at different steps of the metastatic dissemination, the ability of HT1080 cells to activate pro-MMP-2 in the presence of type IV collagen might represent a key regulatory mechanism for the acquisition of an invasive potential.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Maquoi, Erik ; Université de Liège - ULiège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement

Frankenne, F.

Noël, Agnès ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie cellulaire et moléculaire appliquée à l'homme

Krell, H. W.

Grams, F.

Foidart, Jean-Michel ; Université de Liège - ULiège > Département des sciences cliniques > Gynécologie - Obstétrique

Language :

English

Title :

Type Iv Collagen Induces Matrix Metalloproteinase 2 Activation in Ht1080 Fibrosarcoma Cells

Publication date :

15 December 2000

Journal title :

Experimental Cell Research

ISSN :

0014-4827

eISSN :

1090-2422

Publisher :

Academic Press, Orlando, United States - Florida

Volume :

261

Issue :

Pages :

348-59

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 15 May 2009

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277 (2 by ULiège)

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Bibliography

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