Reference : Identifying cows with subclinical mastitis by bulk single nucleotide polymorphism gen...
Scientific journals : Article
Life sciences : Genetics & genetic processes
http://hdl.handle.net/2268/127073
Identifying cows with subclinical mastitis by bulk single nucleotide polymorphism genotyping of tank milk
English
Blard, G. [> >]
Zhang, Zhiyan [Université de Liège - ULg > > > Doct. sc. vété. (Bologne)]
Coppieters, Wouter mailto [Université de Liège - ULg > Département de productions animales > GIGA-R : Génomique animale >]
Georges, Michel mailto [Université de Liège - ULg > Département de productions animales > GIGA-R : Génomique animale >]
2012
Journal of Dairy Science
95
7
4109-13
Yes (verified by ORBi)
International
0022-0302
1525-3198
United States
[en] Alleles ; Animals ; Cattle ; Cell Count/veterinary ; DNA/genetics ; Female ; Genotyping Techniques/veterinary ; Mastitis, Bovine/diagnosis/genetics ; Milk/chemistry/cytology ; Polymorphism, Single Nucleotide/genetics
[en] Mastitis remains the most important health issue in dairy cattle. Improved methods to identify cows developing subclinical mastitis would benefit farmers. We herein describe a novel method to determine the somatic cell counts (SCC) of individual cows by bulk genotyping a sample of milk from the milk tank with panels of genome-wide single nucleotide polymorphisms (SNP). We developed a simple linear model to estimate the contribution of individual cows to the genomic DNA present in the tank milk from 1) the known genotypes of individual cows for the interrogated SNP and 2) the ratio of SNP alleles in the tank milk. Using simulations, we estimate that 3,000, 50,000, and 700,000 SNP are sufficient to accurately (R(2)>0.98) estimate individual SCC in tanks containing milk from 25, 100, and 500 cows, respectively. Using actual data, we demonstrate that the SCC of 21 cows can be estimated with a coefficient of determination of 0.60 using approximately 9,000 SNP. The proposed method increases the value of the proposition of SNP genotyping individual cows for genomic selection purposes.
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/127073
also: http://hdl.handle.net/2268/138583 ; http://hdl.handle.net/2268/138597
Copyright (c) 2012 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

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