Article (Scientific journals)
An ESR, Mass Spectrometry and Fluorescence microscopy approach to study the stearic acid derivatives anchoring in cells
Grammenos, Angeliki; Fillet, Marianne; Collodoro, Mike et al.
2012In Applied Magnetic Resonance
Peer reviewed
 

Files


Full Text
A.Grammenos_revised.doc
Author preprint (2.3 MB)
Download

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
cell; ESR; fluorescence microscopy; mass spectrometry; membranes; 5-DSA
Abstract :
[en] Lateral phase separations in biological membranes are of great interest, making Electron Spin Resonance (ESR) spectroscopy combined with spin labelling a non destructive and sensitive technique for the study of lipid rafts. This is currently accepted that spin probe localization is on the plasma membrane. However, no study confirms this hypothesis. Herein, we report, for the first time, an accurate multi spectral method for the quantification of lipid spin label presence in every sub-cellular fraction. Cells were incubated with 5-doxyl stearic acid derivative and then sub-fractionated. Results of our multimodal spectroscopy approach ubiquitously demonstrate that the presence of ESR spin label only sets in the plasma membranes.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Grammenos, Angeliki ;  Université de Liège - ULiège > Département de physique > Spectroscopie biomédicale
Fillet, Marianne ;  Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments
Collodoro, Mike
Lecart, Sandrine
Quoilin, Caroline ;  Université de Liège - ULiège > Département de Physique > Spectroscopie biomédicale
Fontaine-Aupart, Marie-Pierre
Hoebeke, Maryse  ;  Université de Liège - ULiège > Département de physique > Spectroscopie biomédicale
Language :
English
Title :
An ESR, Mass Spectrometry and Fluorescence microscopy approach to study the stearic acid derivatives anchoring in cells
Publication date :
06 June 2012
Journal title :
Applied Magnetic Resonance
ISSN :
0937-9347
Publisher :
Springer Verlag, Wien, Austria
Peer reviewed :
Peer reviewed
Available on ORBi :
since 07 July 2012

Statistics


Number of views
122 (36 by ULiège)
Number of downloads
158 (7 by ULiège)

Scopus citations®
 
1
Scopus citations®
without self-citations
0
OpenCitations
 
1

Bibliography


Similar publications



Contact ORBi