Reference : An ESR, Mass Spectrometry and Fluorescence microscopy approach to study the stearic a...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/126963
An ESR, Mass Spectrometry and Fluorescence microscopy approach to study the stearic acid derivatives anchoring in cells
English
Grammenos, Angeliki mailto [Université de Liège - ULg > Département de physique > Spectroscopie biomédicale >]
Fillet, Marianne mailto [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
Collodoro, Mike [> >]
Lecart, Sandrine [> >]
Quoilin, Caroline [Université de Liège - ULg > Département de Physique > Spectroscopie biomédicale > >]
Fontaine-Aupart, Marie-Pierre [> >]
Hoebeke, Maryse mailto [Université de Liège - ULg > Département de physique > Spectroscopie biomédicale >]
6-Jun-2012
Applied Magnetic Resonance
Springer Verlag
Yes
International
0937-9347
Wien
Austria
[en] cell ; ESR ; fluorescence microscopy ; mass spectrometry ; membranes ; 5-DSA
[en] Lateral phase separations in biological membranes are of great interest, making Electron Spin Resonance (ESR) spectroscopy combined with spin labelling a non destructive and sensitive technique for the study of lipid rafts. This is currently accepted that spin probe localization is on the plasma membrane. However, no study confirms this hypothesis. Herein, we report, for the first time, an accurate multi spectral method for the quantification of lipid spin label presence in every sub-cellular fraction. Cells were incubated with 5-doxyl stearic acid derivative and then sub-fractionated. Results of our multimodal spectroscopy approach ubiquitously demonstrate that the presence of ESR spin label only sets in the plasma membranes.
http://hdl.handle.net/2268/126963
10.1007/s00723-012-0375-0

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