ORBi: Kaux Jean-François - Effects of platelet-rich plasma (PRP) on the healing of Achilles tendons of rats

Reference : Effects of platelet-rich plasma (PRP) on the healing of Achilles tendons of rats


	Document type :	Scientific journals : Article
	Discipline(s) :	Human health sciences : Orthopedics, rehabilitation & sports medicine
	To cite this reference:	http://hdl.handle.net/2268/126900

Title :	Effects of platelet-rich plasma (PRP) on the healing of Achilles tendons of rats
Language :	English
Author, co-author :	Kaux, Jean-François [Université de Liège - ULg > Département des sciences de la motricité > Département des sciences de la motricité >]
	Drion, Pierre [Université de Liège - ULg > Département des sciences biomédicales et précliniques > GIGA-R:Méth. expér.des anim. de labo et éth. en expér. anim. >]
	Colige, Alain [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Laboratoire des tissus conjonctifs >]
	Pascon, Frédéric [> >]
	Libertiaux, Vincent [> >]
	HOFFMANN, Audrey [Centre Hospitalier Universitaire de Liège - CHU > > Dermatologie >]
	Janssen, Lauriane [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Laboratoire des tissus conjonctifs >]
	HEYERES, Antoine [Centre Hospitalier Universitaire de Liège - CHU > > Dermatologie >]
	Nusgens, Betty [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques >]
	LE GOFF, Caroline [Centre Hospitalier Universitaire de Liège - CHU > > Chimie médicale >]
	Gothot, André [Université de Liège - ULg > Département des sciences cliniques > Département des sciences cliniques >]
	Cescotto, Serge [Université de Liège - ULg > Département ArGEnCo > Département ArGEnCo >]
	Defraigne, Jean-Olivier [Université de Liège - ULg > Département des sciences cliniques > Chirurgie cardio-vasculaire et thoracique >]
	Rickert, Markus [> >]
	Crielaard, Jean-Michel [Université de Liège - ULg > Département des sciences de la motricité > Evaluation et entraînement des aptitudes physiques >]
Publication date :	Sep-2012
Journal title :	Wound Repair & Regeneration : Official Publication of the Wound Healing Society and the European Tissue Repair Society
Publisher :	Blackwell Science
Volume :	20
Issue/season :	5
Pages :	748-756
Peer reviewed :	Yes (verified by ORBi)
Audience :	International
ISSN :	1067-1927
e-ISSN :	1524-475X
City :	Malden
Country :	MA
Keywords :	[en] platelet-rich plasma ; Achilles tendon ; healing ; cryo-jaw ; extracellular matrix proteins
Abstract :	[en] Platelet-Rich Plasma (PRP) contains growth factors involved in the tissular healing process. The aim of the study was to determine if an injection of PRP could improve the healing of sectioned Achilles tendons of rats. After surgery, rats received an injection of PRP (n=60) or a physiological solution (n=60) in situ. After 5, 15 and 30 days, 20 rats of both groups were euthanized and 15 collected tendons were submitted to a biomechanical test using cryo-jaws before performing transcriptomic analyses. Histological and biochemical analyses were performed on the 5 remaining tendons in each group. Tendons in the PRP group were more resistant to rupture at 15 and 30 days. The mechanical stress was significantly increased in tendons of the PRP group at day 30. Histological analysis showed a precocious deposition of fibrillar collagen at day 5 confirmed by a biochemical measurement. The expression of tenomodulin was significantly higher at day 5. The mRNA level of type III collage, matrix metalloproteinase 2, 3 and 9 was similar in the 2 groups at all time points whereas type I collagen was significantly increased at day 30 in the PRP group. In conclusion, an injection of PRP in sectioned rat Achilles tendon influences the early phase of tendons healing and results in an ultimate stronger mechanical resistance.
Permalink :	http://hdl.handle.net/2268/126900
DOI :	10.1111/j.1524-475X.2012.00826.x

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