Reference : Advances in top-down proteomics for disease biomarker discovery.
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/126803
Advances in top-down proteomics for disease biomarker discovery.
English
Calligaris, David [Université de Liège - ULg > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.) >]
Villard, Claude [> >]
Lafitte, Daniel [> >]
Jun-2011
Journal of Proteomics
Elsevier
74
7
920-934
Yes (verified by ORBi)
International
1874-3919
1876-7737
Amsterdam
The Netherlands
[en] Top-down mass spectrometry strategies allow identification and characterization of proteins and protein networks by direct fragmentation. These analytical processes involve a panel of fragmentation mechanisms, some of which preserve protein post-translational modifications. Thus top-down is of special interest in clinical biochemistry to probe modified proteins as potential disease biomarkers. This review describes separating methods, mass spectrometry instrumentation, bioinformatics, and theoretical aspects of fragmentation mechanisms used for top-down analysis. The biological interest of this strategy is extensively reported regarding the characterization of post-translational modifications in biochemical pathways and the discovery of biomarkers. One has to bear in mind that quantitative aspects that are beyond the focus of this review are also of critical important for biomarker discovery. The constant evolution of technologies makes top-down strategies crucial players in clinical and basic proteomics.
Researchers ; Professionals ; Students ; General public
http://hdl.handle.net/2268/126803
10.1016/j.jprot.2011.03.030
http://www.sciencedirect.com/science/article/pii/S1874391911001370

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