Article (Scientific journals)
Advances in top-down proteomics for disease biomarker discovery.
Calligaris, David; Villard, Claude; Lafitte, Daniel
2011In Journal of Proteomics, 74 (7), p. 920-934
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Abstract :
[en] Top-down mass spectrometry strategies allow identification and characterization of proteins and protein networks by direct fragmentation. These analytical processes involve a panel of fragmentation mechanisms, some of which preserve protein post-translational modifications. Thus top-down is of special interest in clinical biochemistry to probe modified proteins as potential disease biomarkers. This review describes separating methods, mass spectrometry instrumentation, bioinformatics, and theoretical aspects of fragmentation mechanisms used for top-down analysis. The biological interest of this strategy is extensively reported regarding the characterization of post-translational modifications in biochemical pathways and the discovery of biomarkers. One has to bear in mind that quantitative aspects that are beyond the focus of this review are also of critical important for biomarker discovery. The constant evolution of technologies makes top-down strategies crucial players in clinical and basic proteomics.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Calligaris, David ;  Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.)
Villard, Claude
Lafitte, Daniel
Language :
English
Title :
Advances in top-down proteomics for disease biomarker discovery.
Publication date :
June 2011
Journal title :
Journal of Proteomics
ISSN :
1874-3919
eISSN :
1876-7737
Publisher :
Elsevier, Amsterdam, Netherlands
Volume :
74
Issue :
7
Pages :
920-934
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 04 July 2012

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