Article (Scientific journals)
Endotoxin-induced basal respiration alterations of renal HK-2 cells: A sign of pathologic metabolism down-regulation
Quoilin, Caroline; Mouithys-Mickalad, Ange; Duranteau, Jacques et al.
2012In Biochemical and Biophysical Research Communications, 423 (2), p. 350-354
Peer reviewed
 

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Keywords :
Sepsis; Inflammation; Acute kidney injury (AKI); Endotoxin; Oxygen consumption; Metabolism
Abstract :
[en] To study the mechanism of oxygen regulation in inflammation-induced acute kidney injury, we investigate the effects of a bacterial endotoxin (lipopolysaccharide, LPS) on the basal respiration of proximal tubular epithelial cells (HK-2) both by high-resolution respirometry and electron spin resonance spectroscopy. These two complementary methods have shown that HK-2 cells exhibit a decreased oxygen consumption rate when treated with LPS. Surprisingly, this cellular respiration alteration persists even after the stress factor was removed. We suggested that this irreversible decrease in renal oxygen consumption after LPS challenge is related to a pathologic metabolic down-regulation such as a lack of oxygen utilization by cells.
Disciplines :
Physical, chemical, mathematical & earth Sciences: Multidisciplinary, general & others
Biochemistry, biophysics & molecular biology
Author, co-author :
Quoilin, Caroline ;  Université de Liège - ULiège > Département de physique > Spectroscopie biomédicale
Mouithys-Mickalad, Ange ;  Université de Liège - ULiège > Centre de l'oxygène : Recherche et développement (C.O.R.D.)
Duranteau, Jacques
Gallez, Bernard
Hoebeke, Maryse  ;  Université de Liège - ULiège > Département de physique > Spectroscopie biomédicale
Language :
English
Title :
Endotoxin-induced basal respiration alterations of renal HK-2 cells: A sign of pathologic metabolism down-regulation
Publication date :
June 2012
Journal title :
Biochemical and Biophysical Research Communications
ISSN :
0006-291X
eISSN :
1090-2104
Publisher :
Academic Press, San Diego, United States - California
Volume :
423
Issue :
2
Pages :
350-354
Peer reviewed :
Peer reviewed
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
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