[en] Several reports have described an association between the presence of soluble human leukocyte antigen G (sHLA-G) in human embryo culture supernatants (ES) and implantation success. However, not all studies agree with these findings. To further document this debate, a multicentre blinded study was performed to investigate, on a large number of IVF ES and ICSI ES, whether sHLA-G is a useful criterion for embryo selection before transfer. A total of 1405 ES from 355 patients were collected from three assisted reproductive technique (ART) centres and evaluated for their sHLA-G content in a single laboratory, using a chemiluminescence enzyme-linked immunosorbent assay. In only one centre was a significant association between sHLA-G-positive ES and successful implantation established (P = 0.0379), whereas no such association was observed in the other centres. It was found that the percentages and concentrations of sHLA-G-positive ES varied between centres, depending on culture media and ART conditions. The percentage of sHLA-G-positive ES was significantly higher in IVF ES than ICSI ES (P < 0.001 and P < 0.01 for two centres). These data demonstrate that substantial variations of sHLA-G content in ES occur between different ART centres, highlighting the influence of several technical parameters that differ from one centre to another.
Disciplines :
Reproductive medicine (gynecology, andrology, obstetrics)
Thonon, Fabienne ; Centre Hospitalier Universitaire de Liège - CHU > Gynécologie-Obstétrique CHR
Parinaud, J.
Léandri, R.
Foidart, Jean-Michel ; Université de Liège - ULiège > Département des sciences cliniques > Gynécologie - Obstétrique - Labo de biologie des tumeurs et du développement
Chaouat, G.
Munaut, Carine ; Université de Liège - ULiège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement
Lombroso, R.
Selva, J.
Bergère, M.
Hammoud, I.
Kozma, N.
Aguerre-Girr, M.
Swales, A. K.
Sargent, I. L.
Le Bouteiller, P.
Lédée, Nathalie ; Université de Liège - ULiège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement
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