Reference : An Improved Protocol for Efficient Engraftment in NOD/ LTSZ-SCIDIL-2RcNULL Mice Allows H...
Scientific journals : Article
Life sciences : Microbiology
http://hdl.handle.net/2268/126403
An Improved Protocol for Efficient Engraftment in NOD/ LTSZ-SCIDIL-2RcNULL Mice Allows HIV Replication and Development of Anti-HIV Immune Responses
English
Singh, Maneesh [> >]
Singh [> >]
Gaudray [> >]
Musumeci, Lucia mailto [Université de Liège - ULg > Département des sciences cliniques > GIGA-R:Immunopath. - Maladies infect. et médec. inter. gén. >]
Thielen, Caroline mailto [Université de Liège - ULg > > Centre de recherches du cyclotron >]
VAIRA, Dolorès mailto [Centre Hospitalier Universitaire de Liège - CHU > > Microbiologie médicale >]
Vandergeeten, Claire [> >]
Delacroix, Laurence mailto [Université de Liège - ULg > Département des sciences cliniques > GIGA-R:Immunopath. - Maladies infect. et médec. inter. gén. >]
Van Gulck, Ellen [> >]
Vanham, Guido [> >]
de Leval, Laurence [> >]
Rahmouni, Souad mailto [Université de Liège - ULg > Département des sciences cliniques > GIGA-R:Immunopath. - Maladies infect. et médec. inter. gén. >]
Moutschen, Michel mailto [Université de Liège - ULg > Département des sciences cliniques > GIGA-R:Immunopath. - Maladies infect. et médec. inter. gén. >]
2012
PLoS ONE
Public Library of Science
7
6
e38491
Yes (verified by ORBi)
International
1932-6203
San Franscisco
CA
[en] Cord blood hematopoietic progenitor cells (CB-HPCs) transplanted immunodeficient NOD/LtsZ-scidIL2Rcnull (NSG) and NOD/SCID/IL2Rcnull (NOG) mice need efficient human cell engraftment for long-term HIV-1 replication studies. Total body
irradiation (TBI) is a classical myeloablation regimen used to improve engraftment levels of human cells in these humanized
mice. Some recent reports suggest the use of busulfan as a myeloablation regimen to transplant HPCs in neonatal and adult
NSG mice. In the present study, we further ameliorated the busulfan myeloablation regimen with fresh CB-CD34+cell
transplantation in 3–4 week old NSG mice. In this CB-CD34+transplanted NSG mice engraftment efficiency of human
CD45+cell is over 90% in peripheral blood. Optimal engraftment promoted early and increased CD3+T cell levels, with better lymphoid tissue development and prolonged human cell chimerism over 300 days. These humanized NSG mice have shown long-lasting viremia after HIV-1JRCSF and HIV-1Bal inoculation through intravenous and rectal routes. We also saw a
gradual decline of the CD4+T cell count, widespread immune activation, up-regulation of inflammation marker and
microbial translocation after HIV-1 infection. Humanized NSG mice reconstituted according to our new protocol produced,
moderate cellular and humoral immune responses to HIV-1 postinfection. We believe that NSG mice reconstituted
according to our easy to use protocol will provide a better in vivo model for HIV-1 replication and anti-HIV-1 therapy trials.
PAI
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http://hdl.handle.net/2268/126403

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