Reference : An improved protocol for efficient engraftment in NOD/LTSZ-SCIDIL-2Rgammanull mice al...
Scientific journals : Article
Human health sciences : Immunology & infectious disease
http://hdl.handle.net/2268/126403
An improved protocol for efficient engraftment in NOD/LTSZ-SCIDIL-2Rgammanull mice allows HIV replication and development of anti-HIV immune responses.
English
Singh, Maneesh [> >]
Singh, Pratibha [> >]
Gaudray, Gilles [> >]
Musumeci, Lucia mailto [Université de Liège - ULg > Département des sciences cliniques > GIGA-R:Immunopath. - Maladies infect. et médec. inter. gén. >]
Thielen, Caroline mailto [Université de Liège - ULg > > GIGA - Neurosciences >]
VAIRA, Dolorès mailto [> >]
Vandergeeten, Claire [> >]
Delacroix, Laurence mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques >]
Van Gulck, Ellen [> >]
Vanham, Guido [> >]
de Leval, Laurence [> >]
Rahmouni, Souad mailto [Université de Liège - ULg > Département des sciences cliniques > GIGA-R:Immunopath. - Maladies infect. et médec. inter. gén. >]
MOUTSCHEN, Michel mailto [Centre Hospitalier Universitaire de Liège - CHU > > Maladies infectieuses et médecine interne générale >]
2012
PLoS ONE
Public Library of Science
7
6
e38491
Yes (verified by ORBi)
International
1932-6203
1932-6203
San Franscisco
United States
[en] Animals ; Antigens, CD34/metabolism ; Antigens, CD45/metabolism ; Cell Differentiation/immunology ; Cell Lineage ; Cell Proliferation ; Cord Blood Stem Cell Transplantation/methods ; Disease Progression ; HIV/immunology/physiology ; HIV Infections/immunology/pathology/virology ; Hematopoietic Stem Cells/metabolism ; Humans ; Immunity/immunology ; Interleukin Receptor Common gamma Subunit/deficiency/immunology ; Lymphoid Tissue/immunology ; Mice ; Mice, Inbred NOD ; Mice, Knockout ; Mice, SCID ; Receptors, Antigen, T-Cell/metabolism ; Receptors, CCR5/metabolism ; Receptors, CXCR4/metabolism ; T-Lymphocytes/immunology/pathology/virology ; Virus Replication/immunology
[en] Cord blood hematopoietic progenitor cells (CB-HPCs) transplanted immunodeficient NOD/LtsZ-scidIL2Rgamma(null) (NSG) and NOD/SCID/IL2Rgamma(null) (NOG) mice need efficient human cell engraftment for long-term HIV-1 replication studies. Total body irradiation (TBI) is a classical myeloablation regimen used to improve engraftment levels of human cells in these humanized mice. Some recent reports suggest the use of busulfan as a myeloablation regimen to transplant HPCs in neonatal and adult NSG mice. In the present study, we further ameliorated the busulfan myeloablation regimen with fresh CB-CD34+cell transplantation in 3-4 week old NSG mice. In this CB-CD34+transplanted NSG mice engraftment efficiency of human CD45+cell is over 90% in peripheral blood. Optimal engraftment promoted early and increased CD3+T cell levels, with better lymphoid tissue development and prolonged human cell chimerism over 300 days. These humanized NSG mice have shown long-lasting viremia after HIV-1JRCSF and HIV-1Bal inoculation through intravenous and rectal routes. We also saw a gradual decline of the CD4+T cell count, widespread immune activation, up-regulation of inflammation marker and microbial translocation after HIV-1 infection. Humanized NSG mice reconstituted according to our new protocol produced, moderate cellular and humoral immune responses to HIV-1 postinfection. We believe that NSG mice reconstituted according to our easy to use protocol will provide a better in vivo model for HIV-1 replication and anti-HIV-1 therapy trials.
PAI
Researchers ; Professionals ; Students ; General public ; Others
http://hdl.handle.net/2268/126403
also: http://hdl.handle.net/2268/160317
10.1371/journal.pone.0038491

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Open access
journal.pone.0038491.pdfPublisher postprint1.34 MBView/Open

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.