Article (Scientific journals)
Overexpression of CD39 in mouse airways promotes bacteria induced inflammation
Theatre, Emilie; Frederix, Kim; Guilmain, William et al.
2012In Journal of Immunology, 189 (4), p. 1966-1974
Peer Reviewed verified by ORBi
 

Files


Full Text
44317_1_merged_1337787837.pdf
Author postprint (30.4 MB)
Download

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
transgenic mice; airway inflammation; bacteria infection
Abstract :
[en] In airways, the ecto-nucleoside triphosphate diphosphohydrolase CD39 plays a central role in the regulation of physiological mucosal nucleotide concentrations and likely contributes to the control of inflammation because accelerated ATP metabolism occurs in chronic inflammatory lung diseases.We sought to determine whether constant elevated CD39 activity in lung epithelia is sufficient to cause inflammation and whether this affects the response to acute LPS or Pseudomonas aeruginosa exposure. We generated transgenic mice overexpressing human CD39 under the control of the airway-specific Clara cell 10-kDa protein gene promoter. Transgenic mice did not develop any spontaneous lung inflammation. However, intratracheal instillation of LPS resulted in accelerated recruitment of neutrophils to the airways of transgenic mice. Macrophage clearance was delayed, and the amounts of CD8+ T and B cells were augmented. Increased levels of keratinocyte chemoattractant, IL-6, and RANTES were produced in transgenic lungs. Similarly, higher numbers of neutrophils and macrophages were found in the lungs of transgenic mice infected with P. aeruginosa, which correlated with improved bacteria clearance. The transgenic phenotype was partially and differentially restored by coinstillation of P2X1 or P2X7 receptor antagonists or of caffeine with LPS. Thus, a chronic increase of epithelial CD39 expression and activity promotes airway inflammation in response to bacterial challenge by enhancing P1 and P2 receptor activation.
Research center :
Giga-Cardiovascular Sciences
Disciplines :
Immunology & infectious disease
Author, co-author :
Theatre, Emilie ;  Université de Liège - ULiège > Sciences Biomédicales et Précliniques > Génétique Humaine
Frederix, Kim;  Université de Liège - ULiège > Sciences Biomédicales et Précliniques > Génétique Humaine
Guilmain, William;  Université de Liège - ULiège > Sciences Biomédicales et Précliniques > Génétique Humaine
Delierneux, Céline ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > GIGA-R : Génétique humaine
Lecut, Christelle ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > GIGA-R : Génétique humaine
Bettendorff, Lucien  ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie humaine et pathologique
BOURS, Vincent ;  Centre Hospitalier Universitaire de Liège - CHU > Génétique
Oury, Cécile  ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > GIGA-R : Génétique humaine
Language :
English
Title :
Overexpression of CD39 in mouse airways promotes bacteria induced inflammation
Alternative titles :
[fr] Une augmentation de l'expression de CD39 dans les voies respiratoires murines favorise l'inflammation en réponse à une infection bactérienne
Publication date :
August 2012
Journal title :
Journal of Immunology
ISSN :
0022-1767
eISSN :
1550-6606
Publisher :
American Association of Immunologists, Baltimore, United States - Maryland
Volume :
189
Issue :
4
Pages :
1966-1974
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 26 June 2012

Statistics


Number of views
104 (12 by ULiège)
Number of downloads
302 (2 by ULiège)

Scopus citations®
 
20
Scopus citations®
without self-citations
20
OpenCitations
 
20

Bibliography


Similar publications



Contact ORBi