ORBi: Hubert Pascale - Local applications of GM-CSF induce the recruitment of immune cells in cervical low-grade squamous intraepithelial lesions.

Reference : Local applications of GM-CSF induce the recruitment of immune cells in cervical low-grad...


	Document type :	Scientific journals : Article
	Discipline(s) :	Human health sciences : Oncology Human health sciences : Immunology & infectious disease
	To cite this reference:	http://hdl.handle.net/2268/1257

Title :	Local applications of GM-CSF induce the recruitment of immune cells in cervical low-grade squamous intraepithelial lesions.
Language :	English
Author, co-author :	Hubert, Pascale [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
	Doyen, Jean [Université de Liège - ULg > > Gynécologie-Obstétrique CHR >]
	Capelle, Xavier [Université de Liège - ULg > > Gynécologie-Obstétrique >]
	Arafa, M. [> >]
	Renoux, Virginie [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
	Bisig, Bettina [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
	Seidel, Laurence [Université de Liège - ULg > Département des sciences de la santé publique > Informatique médicale et biostatistique >]
	Evrard, Brigitte [Université de Liège - ULg > Département de pharmacie > Pharmacie galénique >]
	Bousarghin, Latifa [> >]
	Gerday, Colette [Université de Liège - ULg > > Gynécologie-Obstétrique CHR >]
	Boniver, Jacques [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
	Foidart, Jean-Michel [Université de Liège - ULg > Département des sciences cliniques > Gynécologie - Obstétrique - Labo de biologie des tumeurs et du développement >]
	Delvenne, Philippe [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
	Jacobs, Nathalie [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
Publication date :	2010
Journal title :	American Journal of Reproductive Immunology
Publisher :	Blackwell
Volume :	64
Issue/season :	2
Pages :	126-136
Audience :	International
ISSN :	1046-7408
City :	Oxford
Country :	United Kingdom
Keywords :	[en] Clinical trial ; immunotherapy ; GM-CSF ; Human papillomavirus ; uterine cervical cancer ; adaptive immune response
Abstract :	[en] Abstract Problem Quantitative alterations of antigen-presenting cells (APC) in (pre)neoplastic lesions of the uterine cervix associated with human papillomavirus (HPV) infection suggest a diminished capacity to capture viral antigens and to induce a protective immune response. Method of study To test if a cervical application of GM-CSF could restore an immune response against HPV in women with cervical low-grade squamous intraepithelial lesions (LSIL). We performed two clinical trials with11 healthy women and 15 patients with LSIL. Results GM-CSF applications were well tolerated in all enrolled women and no difference in toxicity between the treated and placebo groups was observed during the follow up (until 30 months). Interestingly, in the GM-CSF treated group, a significant increased APC and cytotoxic T lymphocyte infiltration was observed in the cervical biopsies with no change in regulatory T cell numbers. All the HPV16+ patients exhibited an immune response against HPV16 after GM-CSF applications, as shown by NK and/or T cells producing IFN-γ whereas no cellular immune response was observed before the treatment. Moreover, the anti-VLP antibody titers also increased after the treatment. Conclusion These encouraging results obtained from a limited number of subjects justify further study on the therapeutic effect of APC in cervical (pre)neoplastic lesions.
Research units :	Giga-Infection, Immunity and Inflammation ; Giga-Cancer
Funders :	Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS ; Marshall Program of the Walloon Region ; Fonds Léon Fredericq
Name of the research project :	Colvir (Grant from Walloon Region)
Target :	Researchers ; Professionals ; Students
Permalink :	http://hdl.handle.net/2268/1257
DOI :	10.1111/j.1600-0897.2010.00834.x
Mentions required by the publisher for OA :	The definitive version is available at www3.interscience.wiley.com

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	File	Version	Size	Access
Open access	GM-CSF AJRI.pdf	Author preprint	192.05 kB	View/Open
Open access	Figure GM-CSF.pdf	Author preprint	671.54 kB	View/Open
Open access	Table 1GM-CSF.pdf	Author preprint	46.3 kB	View/Open
Restricted access	Hubert2010American journal of reproductive immunology (New York NY 1989).pdf	Publisher postprint	385.72 kB	Request copy

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