ORBi: Louvet Jean-Noël - Assessment of erythromycin toxicity on activated sludge via batch experiments and microscopic techniques (epifluorescence and CLSM)

Reference : Assessment of erythromycin toxicity on activated sludge via batch experiments and micros...


	Document type :	Scientific journals : Article
	Discipline(s) :	Engineering, computing & technology : Chemical engineering
	To cite this reference:	http://hdl.handle.net/2268/125391

Title :	Assessment of erythromycin toxicity on activated sludge via batch experiments and microscopic techniques (epifluorescence and CLSM)
Language :	English
Author, co-author :	Louvet, Jean-Noël [LRGP (UPR 3349 CNRS) Nancy Université INPL > > > >]
	Helouin, Yannick [LRGP (UPR 3349 CNRS) Nancy Université INPL > > > >]
	Attik, Ghania [LRGP (UPR 3349 CNRS) Nancy Université INPL > > > >]
	Dumas, Dominique [Nancy-Université, Imaging Facility, FR 3209, 7561 CNRS, Faculty of Medicine, > > > >]
	Potier, Olivier [LRGP (UPR 3349 CNRS) Nancy Université INPL > > > >]
	Pons, Marie-Noëlle [ > > ]
Publication date :	2010
Journal title :	Process Biochemistry
Publisher :	Elsevier Science
Volume :	45
Pages :	1187-1794
Audience :	International
ISSN :	1359-5113
City :	Oxford
Country :	United Kingdom
Keywords :	[en] Macrolides ; Antibiotic ; Inhibition ; BacLight ; Microscopy
Abstract :	[en] This study investigates erythromycin toxicity toward activated sludge as a function of exposure time and antibiotic concentration. Batch experiments were conducted and microscopic techniques ranging from bright-ﬁeld microscopy to epiﬂuorescence and confocal laser scanning microscopy (CLSM), combined with a ﬂuorescent viability indicator (BacLight ® Bacterial Viability Kit, Molecular Probes), allowed us to study erythromycin time-kill activity. The erythromycin toxicity was observed at lower concentration when exposure time increased. A 4 .g/L erythromycin concentration was toxic to heterotrophic bacteria on a 5-day time exposure, and a 5 mg/L concentration inhibited nitriﬁcation. These ﬁndings are in agreement with the microscopic studies, which showed a latency time before the lower antibiotic concentrations began to kill bacteria. Microscope slide wells were used as micro-reactors in which erythromycin concentration ranged from 0.1 to 1 mg/L. After 45 min there were 94% (SD 3.8) of living bacteria in control micro-reactors, 67% (SD 3.1) in micro-reactors that contained 0.1 mg/L erythromycin and 37% (SD 18.6) in micro-reactors that contained 1 mg/L erythromycin. CLSM allowed visualization of isolated stained cells in the three-dimensional structure of damaged ﬂocs
Research units :	CNRS
Funders :	Agence Nationale de la Recherche
Target :	Researchers ; Professionals ; Students ; General public
Permalink :	http://hdl.handle.net/2268/125391

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