Reference : Immunocytochemical evidence for production of luteinizing hormone and follicle-stimulati...
Scientific journals : Article
Human health sciences : Endocrinology, metabolism & nutrition
http://hdl.handle.net/2268/1251
Immunocytochemical evidence for production of luteinizing hormone and follicle-stimulating hormone in separate cells in the bovine.
English
Bastings, E. [ > > ]
Beckers, Albert mailto [Université de Liège - ULg > Département des sciences cliniques > Endocrinologie >]
Reznik, Michel [Université de Liège - ULg > Services généraux (Faculté de médecine) > Relations académiques et scientifiques (Médecine) >]
Beckers, Jean-François [Université de Liège - ULg > Département de sciences fonctionnelles > Physiologie de la reproduction >]
1991
Biology of Reproduction
Society for the Study of Reproduction
45
5
788-96
Yes (verified by ORBi)
International
0006-3363
1529-7268
Madison
WI
[en] Animals ; Cattle ; Cytoplasmic Granules/chemistry ; Female ; Follicle Stimulating Hormone/analysis/biosynthesis ; Immunohistochemistry ; Luteinizing Hormone/analysis/biosynthesis ; Male ; Microscopy, Electron ; Pituitary Gland/chemistry/ultrastructure
[en] In all mammalian females, follicular growth and maturation are essentially dependent on the pituitary gonadotropins, FSH and LH. These glycoprotein hormones have many similarities, but their action, based on high affinity binding to specific membrane receptors, are quite different. The purpose of this study was to perform a sensitive localization of FSH and LH in secretory granules of gonadotrophs using highly specific antisera. This morphological study included light microscopy (PAP) and electron microscopy (immunogold single and double labeling) procedures. Histologically, approximatively 11.5% of cells were positive for LH, whereas only 5.4% of cells were positive for FSH. With the electron microscope, single labeling allowed identification of morphologically distinct LH-containing cells and FSH-containing cells. Double immunostaining confirmed that no cells contained both hormones. The finding that FSH and LH are produced in separate pituitary cells is in agreement with recent studies that have suggested a specific role and regulatory process for gonadotropins in the bovine species.
Laboratoire d'Endocrinologie et de Physiologie de la Reproduction animale
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/1251
also: http://hdl.handle.net/2268/5796

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