Article (Scientific journals)
Properties of a class C beta-lactamase from Serratia marcescens.
Joris, Bernard; De Meester, F; Galleni, Moreno et al.
1986In Biochemical Journal, 239 (3), p. 581-6
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Keywords :
Amino Acids/analysis; Bacterial Proteins; Binding Sites; Hydrogen-Ion Concentration; Kinetics; Osmolar Concentration; Peptide Fragments/analysis; Serratia marcescens/enzymology; beta-Lactamases/isolation & purification/metabolism
Abstract :
[en] A beta-lactamase produced by a penicillin-resistant strain of Serratia marcescens was isolated and purified. The kcat. value for benzylpenicillin was about 5% of that observed for the best cephalosporin substrates. However, the low Km of the penam resulted in a high catalytic efficiency (kcat./Km) and the classification of the enzyme as a cephalosporinase might not be completely justified. It also exhibited a low but measurable activity against cefotaxime, cefuroxime, cefoxitin and moxalactam. Substrate-induced inactivation was observed both with a very good (cephalothin) or a very bad (moxalactam) substrate. The active site was labelled by beta-iodopenicillanate. Trypsin digestion produced a 19-residue active-site peptide whose sequence clearly allowed the classification of the enzyme as a class C beta-lactamase.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Joris, Bernard ;  Université de Liège - ULiège > Département des sciences de la vie > Physiologie et génétique bactériennes
De Meester, F
Galleni, Moreno ;  Université de Liège - ULiège > Département des sciences de la vie > Macromolécules biologiques
Masson, Séverine ;  Université de Liège - ULiège > Département de droit > Département de droit
Dusart, Jean;  Université de Liège - ULiège > Services administratifs généraux > R&D : Gestion opérationnelle
Frère, Jean-Marie ;  Université de Liège - ULiège > Centre d'ingénierie des protéines
Van Beeumen, J
Bush, K
Sykes, R
Language :
English
Title :
Properties of a class C beta-lactamase from Serratia marcescens.
Publication date :
1986
Journal title :
Biochemical Journal
ISSN :
0264-6021
eISSN :
1470-8728
Publisher :
Portland Press, London, United Kingdom
Volume :
239
Issue :
3
Pages :
581-6
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 12 June 2012

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