Article (Scientific journals)
The beta-lactamase of Enterobacter cloacae P99. Chemical properties, N-terminal sequence and interaction with 6 beta-halogenopenicillanates.
Joris, Bernard; De Meester, F; Galleni, Moreno et al.
1985In Biochemical Journal, 228 (1), p. 241-8
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Keywords :
Amino Acid Sequence; Amino Acids/analysis; Enterobacter/enzymology; Enterobacteriaceae/enzymology; Kinetics; Penicillanic Acid/metabolism; Spectrophotometry; Tryptophan/analysis; beta-Lactamases/antagonists & inhibitors
Abstract :
[en] The beta-lactamase of Enterobacter cloacae P99 consists of one polypeptide chain of Mr 39000 devoid of disulphide bridges and free thiol groups. It contains an unusually high proportion of tyrosine and tryptophan. The N-terminal sequence exhibits overlaps with the tryptic peptide obtained after labelling the active site with 6 beta-iodopenicillanate. The active-site serine residue is at position 64. The homology with the chromosomal beta-lactamase of Escherichia coli K 12 (ampC gene) is lower within the 25 residues of the N-terminal portion than around the active-site serine residue. The P99 beta-lactamase is inactivated by 6 beta-bromo- and 6 beta-iodo-penicillanate, with a second-order rate constant of 110-140M-1 X s-1 at 30 degrees C and pH 7.0, a value that is much lower than that observed with class-A beta-lactamases.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Joris, Bernard ;  Université de Liège - ULiège > Département des sciences de la vie > Physiologie et génétique bactériennes
De Meester, F
Galleni, Moreno ;  Université de Liège - ULiège > Département des sciences de la vie > Macromolécules biologiques
Reckinger, G
Coyette, Jacques ;  Université de Liège - ULiège > Services généraux (Faculté des sciences) > Relations académiques et scientifiques (Sciences)
Frère, Jean-Marie ;  Université de Liège - ULiège > Centre d'ingénierie des protéines
Van Beeumen, J
Language :
English
Title :
The beta-lactamase of Enterobacter cloacae P99. Chemical properties, N-terminal sequence and interaction with 6 beta-halogenopenicillanates.
Publication date :
1985
Journal title :
Biochemical Journal
ISSN :
0264-6021
eISSN :
1470-8728
Publisher :
Portland Press, London, United Kingdom
Volume :
228
Issue :
1
Pages :
241-8
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 12 June 2012

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