Reference : Use of semi-quantitative kit methods to study the heterotrophic bacterial community o...
Scientific journals : Article
Physical, chemical, mathematical & earth Sciences : Earth sciences & physical geography
http://hdl.handle.net/2268/124736
Use of semi-quantitative kit methods to study the heterotrophic bacterial community of Posidonia oceanica meadows: Limits and possible applications
English
Richir, Jonathan mailto [Université de Liège - ULg > Département de Biologie, Ecologie et Evolution > Océanologie >]
Velimirov, Branko [> >]
Poulicek, Mathieu mailto [Université de Liège - ULg > Département de Biologie, Ecologie et Evolution > Ecologie animale et écotoxicologie >]
Gobert, Sylvie mailto [Université de Liège - ULg > Département de Biologie, Ecologie et Evolution > Océanologie >]
2012
Estuarine Coastal & Shelf Science
Academic Press
109
20-29
Yes (verified by ORBi)
International
0272-7714
London
United Kingdom
[en] heterotroph bacteria ; Posidonia oceanica ; semi-quantitative kit assays
[en] Rapid, easy and low cost semi-quantitative methods were tested to study the heterotrophic bacterial community of Posidonia oceanica meadows and were compared to techniques commonly used in microbial ecology. Free and pore-water bacterial densities were estimated by luminescence, and principal enzymatic activities, metabolic capabilities and benthic mineralisation processes were studied with microtitration methods: ApiZym galleries, Biolog microplates and BART tests. Bacterial densities varied little throughout the year and were around 5.0 10^5 and 6.0 10^6 cells ml -1 of free and pore-water, respectively. The combined use of the ApiZym gallery and the Biolog microtitration plate permitted highlighting bacterial enzymatic activities susceptible to degrade principal organic polymers present in the Posidonia meadow, and to correlate these enzymatic activities to the subsequent potential utilization of resulting monomeric products. Levels of enzymatic activities (1.80-8.36 nmoles substrates h -1 ml- 1) and energetic bacterial metabolism (1.80-6.42 nmoles substrates h -1 ml- 1) presented seasonality relying on the temperature regime and on the primary production (Posidonia and phytoplankton). Main mineralization processes of buried organic matter through sulfate and iron reduction activities were successfully detected. Despite the complexity of the studied ecosystem, results obtained by this semi-quantitative approach, compared to studies applying commonly used methods in microbial ecology, highlighted the same bacterial dominant key processes. Their low cost, rapid and easy use, and the low level of expertise and sophistication they require means that these techniques are of use to many employed in environmental surveys.
Laboratoire d'Océanologie
French Community of Belgium (ARC RACE 05/10-333 and FC 19442 / 2.4.502.08 F)
RACE
Researchers
http://hdl.handle.net/2268/124736
http://www.journals.elsevier.com/estuarine-coastal-and-shelf-science/

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