Reference : Sperm quality analysis in XX, XY and YY males of the Nile tilapia (Oreochromis niloticus).
Scientific journals : Article
Life sciences : Zoology
http://hdl.handle.net/2268/124169
Sperm quality analysis in XX, XY and YY males of the Nile tilapia (Oreochromis niloticus).
English
Gennotte, Vincent mailto [Université de Liège - ULg > > Centre de formation et de recherche en aquaculture (CEFRA)]
François, Ekniel [Caribbean Harvest, Impasse Dessources 2, Vivy Mitchel-Petion Ville, Haiti > > > >]
Rougeot, Carole mailto [Université de Liège - ULg > > Centre de formation et de recherche en aquaculture (CEFRA)]
Ponthier, Jérôme mailto [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux]
Deleuze, Stefan mailto [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Obstét. et path. de la reprod. des anim. de comp. et équidés]
Mélard, Charles mailto [Université de Liège - ULg > > Centre de formation et de recherche en aquaculture (CEFRA) >]
2012
Theriogenology
Elsevier
Yes
International
0093-691X
New York
NY
[en] Oreochromis niloticus ; sperm motility ; sperm quality ; CASA ; sexual genotype ; tilapia
[en] In Nile tilapia (Oreochromis niloticus), individuals with atypical sexual genotype are commonly used in farming (use of YY males to produce all-male offsprings), but they also constitute major tools to study sex determinism mechanisms. In other species, sexual genotype and sex reversal procedures affect different aspects of biology such as growth, behaviour and reproductive success. The aim of this study was to assess the influence of sexual genotype on sperm quality in Nile tilapia. Milt characteristics were compared in XX (sex-reversed), XY and YY males in terms of gonadosomatic index, sperm count, sperm motility and duration of sperm motility. Sperm motility was measured by computer-assisted sperm analysis (CASA) quantifying several parameters: total motility, progressive motility, curvilinear velocity, straight line velocity, average path velocity and linearity. None of the sperm trait measured differed significantly between the three genotypes. Mean values of gonadosomatic index, sperm concentration and sperm motility duration of XX, XY and YY males respectively ranged from 0.92 to 1.33 %, from 1.69 to 2.22 × 10(9) cells mL-1 and from 18’04’’ to 27’32’’. Mean values of total motility and curvilinear velocity 1 min after sperm activation respectively ranged from 53 to 58 % and from 71 to 76 µm s-1 for the three genotypes. After 3 min of activity, all the sperm motility and velocity parameters dropped by half and continued to slowly decrease thereafter. Seven min after activation, only 9 to 13 % of spermatozoa were still progressive. Our results prove that neither sexual genotype nor hormonal sex reversal treatments affect sperm quality in male Nile tilapias with atypical sexual genotype.
Applied and Fundamental FISH Research Center - AFFISH-RC
http://hdl.handle.net/2268/124169
10.1016/j.theriogenology.2012.02.002
http://dx.doi.org/10.1016/j.theriogenology.2012.02.002
Copyright (c) 2012 Elsevier Inc. All rights reserved.

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Restricted access
Gennotte 2012.pdfPublisher postprint836.12 kBRequest copy

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.