Reference : Increasing the Detection Limit of the Parkinson Disorder through a Specific Surface Chem...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/124048
Increasing the Detection Limit of the Parkinson Disorder through a Specific Surface Chemistry Applied onto Inner Surface of the Titration Well
English
Mille, Caroline [> >]
Debarnot [> >]
Zorzi, Willy mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie humaine >]
Elmoualij, Benaïssa mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie humaine >]
Coudreuse, Arnaud [> >]
Legeay, Gilbert [> >]
Quadrio, Isabelle [> >]
Perret-Liaudet, Armand [> >]
Poncin-Epaillard, Fabienne [> >]
Mar-2012
Journal of Functional Biomaterials
MDPI AG, JFB editorial office
2
298-312
Yes (verified by ORBi)
International
2079-4983
Switzerland
[en] Parkinson ; Surfaces ; Surfactants
[en] The main objective of this paper was to illustrate the enhancement of the sensitivity of ELISA titration for neurodegenerative proteins by reducing nonspecific adsorptions that could lead to false positives. This goal was obtained thanks to the association of plasma and wet chemistries applied to the inner surface of the titration well. The polypropylene surface was plasma-activated and then, dip-coated with different amphiphilic molecules. These molecules have more or less long hydrocarbon chains and may be charged. The modified surfaces were characterized in terms of hydrophilic—phobic character, surface chemical groups and topography. Finally, the coated wells were tested during the ELISA titration of the specific antibody capture of the α-synuclein protein. The highest sensitivity is obtained with polar (Θ = 35°), negatively charged and smooth inner surface.
CRPP
FP6
Differential diagnosis of Neurodegeneratives disorders
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/124048
http://www.mdpi.com/2079-4983/3/2/298/

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