ORBi: Collodoro Mike - Identification and quantification of concentration-dependent biomarkers in MCF-7/BOS cells exposed to 17β-estradiol by 2-D DIGE and label-free proteomics

Reference : Identification and quantification of concentration-dependent biomarkers in MCF-7/BOS cel...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Food science Life sciences : Environmental sciences & ecology Physical, chemical, mathematical & earth Sciences : Chemistry
	To cite this reference:	http://hdl.handle.net/2268/124038

Title :	Identification and quantification of concentration-dependent biomarkers in MCF-7/BOS cells exposed to 17β-estradiol by 2-D DIGE and label-free proteomics
Language :	English
Author, co-author :	Collodoro, Mike [Université de Liège - ULg > > > Doct. sc. (bioch., biol. mol.&cell., bioinf.&mod.-Bologne)]
	Lemaire, Pascale [Université de Liège - ULg > > > Doct. sc. (bioch., biol. mol.&cell., bioinf.&mod.-Bologne)]
	Eppe, Gauthier [Université de Liège - ULg > > Chimie analytique inorganique >]
	Bertrand, Virginie [Université de Liège - ULg > > > Doct. sc. (chimie - Bologne)]
	Dobson, Rowan [Université de Liège - ULg > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.) >]
	Mazzucchelli, Gabriel [Université de Liège - ULg > > Center for Analytical Research and Technology (CART) >]
	Widart, Joëlle [Université de Liège - ULg > Département de pharmacie > Département de pharmacie >]
	De Pauw, Edwin [Université de Liège - ULg > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.) >]
	Gillet, Marie-Claire [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie - Cytologie >]
Publication date :	2-May-2012
Journal title :	Journal of Proteomics
Publisher :	Elsevier
Volume :	in press
Audience :	International
ISSN :	1874-3919
e-ISSN :	1876-7737
City :	Amsterdam
Country :	The Netherlands
Keywords :	[en] 17β-estradiol ; MCF-7 BOS ; 2-D DIGE ; Label-free ; SRM ; xenoestrogens
Abstract :	[en] This paper reports the identification of biomarkers resulting from the exposure of MCF-7/BOS cells to 17β-estradiol (E2). The biomarkers were identified using 2 independent and complementary techniques, 2-D DIGE / MALDI-TOF peptide mass fingerprint, and 2-D UPLC-ESI MS/MS. These markers form a preliminary molecular signature that can be used when testing the estrogenic activity of xenobiotics, either pure or in mixtures.
Research units :	Giga-Systems Biology and Chemical Biology ; Centre Interfacultaire d'Analyse des Résidus en Traces - CART
Funders :	Fonds Européen de Développement Régional - FEDER ; Fonds pour la formation à la Recherche dans l'Industrie et dans l'Agriculture (Communauté française de Belgique) - FRIA ; Union Européenne = European Union - UE = EU
Target :	Researchers ; Professionals ; Students
Permalink :	http://hdl.handle.net/2268/124038
DOI :	10.1016/j.jprot.2012.04.032
Other URL :	http://www.sciencedirect.com/science/article/pii/S1874391912002515

File(s) associated to this reference

Fulltext file(s):

	File	Commentary	Version	Size	Access
Restricted access	Collodoro et al.pdf		Publisher postprint	1.51 MB	Request copy

All documents in ORBi are protected by a user license.

Dspace v1.5