Reference : Role of the adaptor protein CIKS in the activation of the IKK complex
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/1240
Role of the adaptor protein CIKS in the activation of the IKK complex
English
Mauro, Claudio [‘Federico II’ University of Naples > Dipartimento di Biologia e Patologia Cellulare e Molecolare]
Vito, Pasquale [Universita degli Studi del Sannio (Benevento) > Dipartimento di Scienze Biologiche e Ambientali]
Mellone, Stefano [CNR (Naples) > Istituto di Endocrinologia e Oncologia Sperimentale]
Pacifico, Francesco [CNR (Naples) > Istituto di Endocrinologia e Oncologia Sperimentale]
Chariot, Alain mailto [Université de Liège - ULg > Département de pharmacie > Chimie médicale >]
Formisano, Silvestro [‘Federico II’ University of Naples > Dipartimento di Biologia e Patologia Cellulare e Molecolare]
Leonardi, Antonio [‘Federico II’ University of Naples > Dipartimento di Biologia e Patologia Cellulare e Molecolare]
12-Sep-2003
Biochemical and Biophysical Research Communications
Academic Press Inc Elsevier Science
309
1
84-90
Yes (verified by ORBi)
International
0006-291X
San Diego
[en] NF-kappa B ; CIKS/Act1 ; NEMO/IKK gamma ; signal transduction
[en] Nuclear factor kappaB (NF-kappaB) plays a pivotal role in numerous cellular processes, including stress response, inflammation, and protection from apoptosis. Therefore, the activity of NF-kappaB needs to be tightly regulated. We have previously identified a novel gene, named CIKS (connection to IkappaB-kinase and SAPK), able to bind the regulatory sub-unit NEMO/IKKgamma and to activate NF-kappaB. Here, we demonstrate that CIKS forms homo-oligomers, interacts with NEMO/IKKgamma, and is recruited to the IKK-complex upon cell stimulation. In addition, we identified the regions of CIKS responsible for these functions. We found that the ability of CIKS to oligomerize, and to be recruited to the IKK-complex is not sufficient to activate the NF-kappaB In fact, a deletion mutant of CIKS able to oligomerize, to interact with NEMO/IKKgamma, and to be recruited to the IKK-complex does not activate NF-kappaB, suggesting that CIKS needs a second level of regulation to efficiently activate NF-kappaB. (C) 2003 Elsevier Inc. All rights reserved.
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/1240

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