endometriosis; in vitro system; adhesion; and cytokines
Abstract :
[en] Objective: To evaluate, in a new original in vitro assay, putative factors that could modulate the adhesion of endometrial cells to peritoneum. Design: Prospective, controlled in vitro study. Setting: Academic research laboratory. Patient(s): Fourteen nonmenopausal women undergoing hysterectomy or laparoscopy for benign gynecologic indication. Intervention(s): Endometrial cells obtained from women with regular cycles without endometriosis were labeled with (111)Indium and confronted in vitro with mouse peritoneum in the presence of various cytokines and/or antiadhesive compounds. Main Outcome Measure(s): Radioactivity in (111)Indium-labeled endometrial cells. Result(s): The adhesion of human endometrial cells to mouse peritoneum was increased by treatment with pro-inflammatory cytokines (interleukin IL-1 beta, IL-6, TNF alpha, TGF-beta1). Whereas heparan sulfate had no effect on cell adhesion, a gel of ferric hyaluronate (Intergel) was able to counteract the pro-adhesive effect of cytokines. Interestingly, the pretreatment of peritoneum with cytokines, 24 hours before cell seeding in the presence of the ferric hyaluronate gel, restored the cytokine-promoting effect on cell adhesion. Conclusion(s): Proinflammatory cytokines promote the in vitro peritoneal adhesion of endometrial cells. An antiadhesive hyaluronate gel used in clinics decreases the adhesion in a dose-dependent manner and reduces cytokine bioavailability. (Fertil Steril((R)) 2003;79(Suppl 1):724-9. (C) 2003 by American Society for Reproductive Medicine.).
Disciplines :
Reproductive medicine (gynecology, andrology, obstetrics)
Author, co-author :
Beliard, Aude ; Centre Hospitalier Universitaire de Liège - CHU > Gynécologie-Obstétrique CHR
Noël, Agnès ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie cellulaire et moléculaire appliquée à l'homme
Goffin, Frédéric ; Centre Hospitalier Universitaire de Liège - CHU > Gynécologie-Obstétrique CHR
Frankenne, Francis
Foidart, Jean-Michel ; Université de Liège - ULiège > Département des sciences cliniques > Gynécologie - Obstétrique
Language :
English
Title :
Adhesion of endometrial cells labeled with 111 Indium-tropolonate to peritoneum: a novel in vitro model to study endometriosis
Sampson J.A. The development of the implantation theory for the origin of peritoneal endometriosis. Am J Obstet Gynecol. 40:1940;449-557.
van der Linden P.J. Theories on the pathogenesis of endometriosis. Hum Reprod. 11:(Suppl 3):1996;53-65.
Barcz E., Kaminski P., Marianowski L. Role of cytokines in pathogenesis of endometriosis. Med Sci Monit. 6:2000;1042-1046.
Zhang R.J., Wild R.A., Ojago J.M. Effect of tumor necrosis factor-alpha on adhesion of human endometrial stromal cells to peritoneal mesothelial cells an in vitro system . Fertil Steril. 59:1993;1196-1201.
Hammond M.G., Oh S.T., Anners J., Surrey E.S., Halme J. The effect of growth factors on the proliferation of human endometrial stromal cells in culture. Am J Obstet Gynecol. 168:1993;1131-1136.
Halme J., Becker S., Haskill S. Altered maturation and function of peritoneal macrophages possible role in pathogenesis of endometriosis . Am J Obstet Gynecol. 156:1987;783-789.
Rana N., Braun D.P., House R., Gebel H., Rotman C., Dmowski W.P. Basal and stimulated secretion of cytokines by peritoneal macrophages in women with endometriosis. Fertil Steril. 65:1996;925-930.
Oosterlynck D.J., Meuleman C., Waer M., Koninckx P.R. Transforming growth factor-beta activity is increased in peritoneal fluid from women with endometriosis. Obstet Gynecol. 83:1994;287-292.
D'Hooghe T.M., Bambra C.S., Xiao L., Peixe K., Hill J.A. Effect of menstruation and intrapelvic injection of endometrium on inflammatory parameters of peritoneal fluid in the baboon (Papio anubis and Papio cynocephalus). Am J Obstet Gynecol. 184:2001;917-925.
Witz C.A., Montoya-Rodriguez I.A., Miller D.M., Schneider B.G., Schenken R.S. Mesothelium expression of integrins in vivo and in vitro. J Soc Gynecol Investig. 5:1998;87-93.
Danpure H.J., Osman S., Brady F. The labelling of blood cells in plasma with 111In-tropolonate. Br J Radiol. 55:1982;247-249.
Motulsky H. Intuitive biostatistics. 1995;Oxford University Press, Oxford, United Kingdom.
Aktolun C., Ussov W.Y., Arka A., Glass D., Gunasekera R.D., Peters A.M. Technetium-99m and indium-111 double labelling of granulocytes for kinetic and clinical studies. Eur J Nucl Med. 22:1995;330-334.
Giannessi D., Bernini W., Puccetti P., De Caterina R. Platelet radiolabelling with 111 Indium for in vivo studies a methodological reappraisal . Nucl Med Biol. 22:1995;399-403.
Somigliana E., Vigano P., Rossi G., Carinelli S., Vignali M., Panina-Bordignon P. Endometrial ability to implant in ectopic sites can be prevented by interleukin-12 in a murine model of endometriosis. Hum Reprod. 14:1999;2944-2950.
Ingelmo J.M., Quereda F., Acien P. Intraperitoneal and subcutaneous treatment of experimental endometriosis with recombinant human interferon-alpha-2b in a murine model. Fertil Steril. 71:1999;907-911.
D'Antonio M., Martelli F., Peano S., Papoian R., Borrelli F. Ability of recombinant human TNF binding protein-1 (r-hTBP-1) to inhibit the development of experimentally-induced endometriosis in rats. J Reprod Immunol. 48:2000;81-98.
Garcia-Velasco J.A., Arici A. Interleukin-8 stimulates the adhesion of endometrial stromal cells to fibronectin. Fertil Steril. 72:1999;336-340.
Wild R.A., Zhang R.J., Medders D. Whole endometrial fragments form characteristics of in vivo endometriosis in a mesothelial cell co-culture system an in vitro model for the study of the histogenesis of endometriosis . J Soc Gynecol Investig. 1:1994;65-68.
Beliard A, Noel A, Goffin F, Frankenne F, Foidart JM. Role of endocrine status and cell type in adhesion of human endometrial cells to the peritoneum in nude mice. Fertil Steril 2002;78:973-8.
Bartosik D., Jacobs S.L., Kelly L.J. Endometrial tissue in peritoneal fluid. Fertil Steril. 46:1986;796-800.
Williams R.S., Rossi A.M., Chegini N., Schultz G. Effect of transforming growth factor beta on postoperative adhesion formation and intact peritoneum. J Surg Res. 52:1992;65-70.
Lucas P.A., Warejcka D.J., Young H.E., Lee B.Y. Formation of abdominal adhesions is inhibited by antibodies to transforming growth factor-beta1. J Surg Res. 65:1996;135-138.
Dou Q., Williams R.S., Chegini N. Inhibition of transforming growth factor-beta 1 alters the growth, anchor-dependent cell aggregation and integrin mRNA expression in human promonocytes implications for endometriosis and peritoneal adhesion formation . Mol Hum Reprod. 3:1997;383-391.