Reference : Murine 5T multiple myeloma cells induce angiogenesis in vitro and in vivo
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/12326
Murine 5T multiple myeloma cells induce angiogenesis in vitro and in vivo
English
Van Valckenborgh, E. [ > > ]
De Raeve, H. [ > > ]
Devy, L. [ > > ]
Blacher, Silvia mailto [Université de Liège - ULg > Département des sciences cliniques > Labo de biologie des tumeurs et du développement >]
Munaut, Carine mailto [Université de Liège - ULg > Département des sciences cliniques > Labo de biologie des tumeurs et du développement >]
Noël, Agnès mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Biologie cellulaire et moléculaire appliquée à l'homme >]
Van Marck, E. [ > > ]
Van Riet, I. [ > > ]
Van Camp, B. [ > > ]
Vanderkerken, K. [ > > ]
2002
British Journal of Cancer
Nature Publishing Group
86
5
796-802
Yes (verified by ORBi)
International
0007-0920
1532-1827
London
United Kingdom
[en] multiple myeloma ; angiogenesis ; rat aortic ring assay ; MVD
[en] Multiple myeloma is a B cell malignancy. Recently, it has been demonstrated that bone marrow samples of patients with multiple myeloma display an enhanced angiogenesis. The mechanisms involved seem to be multiple and complex. We here demonstrate that the murine 5T multiple myeloma models are able to induce angiogenesis in vitro by using a rat aortic ring assay and in vivo by determining the microvessel density. The rat aortic rings cultured in 5T multiple myeloma conditioned medium exhibit a higher number of longer and more branched microvessels than the rings cultured in control medium. In bone marrow samples from 5T multiple myeloma diseased mice, a statistically significant increase of the microvessel density was observed when compared to bone marrow samples from age-matched controls. The angiogenic phenotype of both 5T multiple myeloma cells could be related, at least in part, to their capacity to produce vascular endothelial growth factor. These data clearly demonstrate that the 5T multiple myeloma models are good models to study angiogenesis in multiple myeloma and will allow to unravel the mechanisms of neovascularisation, as well as to test new putative inhibitors of angiogenesis.
http://hdl.handle.net/2268/12326
10.1038/sj.bjc.6600137

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