Article (Scientific journals)
The K1 beta-lactamase of Klebsiella pneumoniae.
Joris, Bernard; De Meester, F; Galleni, Moreno et al.
1987In Biochemical Journal, 243 (2), p. 561-7
Peer Reviewed verified by ORBi
 

Files


Full Text
Publi_BJ_18.pdf
Publisher postprint (3.3 MB)
Download

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
Amino Acids/analysis; Binding Sites; Kinetics; Klebsiella pneumoniae/enzymology; Penicillinase/metabolism; Peptide Fragments/isolation & purification; Peptide Mapping; Spectrophotometry, Ultraviolet; Sulfhydryl Compounds/analysis
Abstract :
[en] beta-Lactamase K1 was purified from Klebsiella pneumoniae SC10436. It is very similar to the enzyme produced by Klebsiella aerogenes 1082E and described by Emanuel, Gagnon & Waley [Biochem. J. (1986) 234, 343-347]. An active-site peptide was isolated after labelling of the enzyme with tritiated beta-iodopenicillanate. A cysteine residue was found just before the active-site serine residue. This result could explain the properties of the enzyme after modification by thiol-blocking reagents. The sequence of the active-site peptide clearly established the enzyme as a class A beta-lactamase.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Joris, Bernard ;  Université de Liège - ULiège > Département des sciences de la vie > Physiologie et génétique bactériennes
De Meester, F
Galleni, Moreno ;  Université de Liège - ULiège > Département des sciences de la vie > Macromolécules biologiques
Frère, Jean-Marie ;  Université de Liège - ULiège > Centre d'ingénierie des protéines
Van Beeumen, J
Language :
English
Title :
The K1 beta-lactamase of Klebsiella pneumoniae.
Publication date :
1987
Journal title :
Biochemical Journal
ISSN :
0264-6021
eISSN :
1470-8728
Publisher :
Portland Press, London, United Kingdom
Volume :
243
Issue :
2
Pages :
561-7
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 21 May 2012

Statistics


Number of views
78 (0 by ULiège)
Number of downloads
106 (0 by ULiège)

Scopus citations®
 
13
Scopus citations®
without self-citations
11
OpenCitations
 
14

Bibliography


Similar publications



Contact ORBi