Reference : Effects of platelet-rich plasma on the healing of tendons: animal model
Scientific congresses and symposiums : Paper published in a book
Human health sciences : Orthopedics, rehabilitation & sports medicine
http://hdl.handle.net/2268/122525
Effects of platelet-rich plasma on the healing of tendons: animal model
English
Kaux, Jean-François mailto [Université de Liège - ULg > Département des sciences de la motricité > Département des sciences de la motricité >]
Drion, Pierre mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > GIGA-R:Méth. expér.des anim. de labo et éth. en expér. anim. >]
Colige, Alain mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Laboratoire des tissus conjonctifs >]
Pascon, Frédéric [> >]
Libertiaux, Vincent [> >]
HOFFMANN, Audrey [Centre Hospitalier Universitaire de Liège / ULg > Dpt des Sciences médicales et précliniques > Laboratoire de Biologie des Tissus Conjonctifs > > >]
Janssen, Lauriane mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Laboratoire des tissus conjonctifs >]
HEYERES, Antoine [Centre Hospitalier Universitaire de Liège / ULg > Dpt des sciences biomédicales et précliniques > Laboratoire de Biologie des Tissus Conjonctifs > > >]
Nusgens, Betty mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Laboratoire de Biologie des Tissus Conjonctifs > > >]
LE GOFF, Caroline mailto [Centre Hospitalier Universitaire de Liège - CHU > > Chimie médicale >]
Gothot, André mailto [Université de Liège - ULg > Département des sciences cliniques > Département des sciences cliniques >]
Cescotto, Serge mailto [Université de Liège - ULg > Département ArGEnCo > Département ArGEnCo >]
Defraigne, Jean-Olivier mailto [Université de Liège - ULg > Département des sciences cliniques > Chirurgie cardio-vasculaire et thoracique >]
Rickert, Markus [> >]
Crielaard, Jean-Michel mailto [Université de Liège - ULg > Département des sciences de la motricité > Evaluation et entraînement des aptitudes physiques >]
Apr-2012
Biomedica 2012
180
Yes
International
Biomedica 2012 - The European Life Sciences Summit
18-19 April 2012
Liège
Belgique
[en] Introduction: Platelet-Rich Plasma (PRP) contains lot of growth factors which could enhance the healing process of different tissues. We aimed to determine if a single injection of PRP could improve the cicatrisation of ruptured Achilles tendons of rats.
Material and Methods: A 5mm defect was surgically made in the Achilles tendon of 120 rats. A few hours after surgery, 45 rats received a PRP or PBS injection in situ. After 5, 15 and 30 days, 20 rats of both groups were euthanized and 15 collected tendons were immediately submitted to a biomechanical tensile strength test until rupture using a “cryo-jaw” device. After, theses samples were used for transcriptomic analyses. Histological and biochemical analyses were performed on the five remained tendons in each group.
Results: Tendons in the PRP group were more resistant to rupture at 15 and 30 days than those in the control group. The transverse area of tendons in the PRP group was significantly higher at day 5 and 15. The constraint was significantly increased in tendons of the PRP group in the late phase of the healing (day 30). Histological and immunohistological analysis showed an increased staining for fibrillar collagen at day 5 confirmed by a biochemical analysis showing an increased collagen concentration in the callus. The expression of tenomodulin, a tenocyte differentiation marker, was significantly higher in the PRP-treated tendons at day 5. No significant difference in terms of mRNA for type III collagen and matrix metalloproteinase 9 was observed at any time between the 2 groups.
Conclusion: A single injection of PRP in sectioned Achilles tendon of rats few hours after surgery influences the early phase of tendons healing, resulting in an ultimate stronger mechanical resistance.
http://hdl.handle.net/2268/122525

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