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Effects of platelet-rich plasma on the healing of tendons: animal model
Kaux, Jean-François; Drion, Pierre; Colige, Alain et al.
2012In Biomedica 2012
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Abstract :
[en] Introduction: Platelet-Rich Plasma (PRP) contains lot of growth factors which could enhance the healing process of different tissues. We aimed to determine if a single injection of PRP could improve the cicatrisation of ruptured Achilles tendons of rats. Material and Methods: A 5mm defect was surgically made in the Achilles tendon of 120 rats. A few hours after surgery, 45 rats received a PRP or PBS injection in situ. After 5, 15 and 30 days, 20 rats of both groups were euthanized and 15 collected tendons were immediately submitted to a biomechanical tensile strength test until rupture using a “cryo-jaw” device. After, theses samples were used for transcriptomic analyses. Histological and biochemical analyses were performed on the five remained tendons in each group. Results: Tendons in the PRP group were more resistant to rupture at 15 and 30 days than those in the control group. The transverse area of tendons in the PRP group was significantly higher at day 5 and 15. The constraint was significantly increased in tendons of the PRP group in the late phase of the healing (day 30). Histological and immunohistological analysis showed an increased staining for fibrillar collagen at day 5 confirmed by a biochemical analysis showing an increased collagen concentration in the callus. The expression of tenomodulin, a tenocyte differentiation marker, was significantly higher in the PRP-treated tendons at day 5. No significant difference in terms of mRNA for type III collagen and matrix metalloproteinase 9 was observed at any time between the 2 groups. Conclusion: A single injection of PRP in sectioned Achilles tendon of rats few hours after surgery influences the early phase of tendons healing, resulting in an ultimate stronger mechanical resistance.
Disciplines :
Orthopedics, rehabilitation & sports medicine
Author, co-author :
Kaux, Jean-François  ;  Université de Liège - ULiège > Département des sciences de la motricité > Département des sciences de la motricité
Drion, Pierre ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > GIGA-R:Méth. expér.des anim. de labo et éth. en expér. anim.
Colige, Alain ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Laboratoire des tissus conjonctifs
Pascon, Frédéric 
Libertiaux, Vincent
HOFFMANN, Audrey ;  Centre Hospitalier Universitaire de Liège / ULiège > Dpt des Sciences médicales et précliniques > Laboratoire de Biologie des Tissus Conjonctifs
Janssen, Lauriane ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Laboratoire des tissus conjonctifs
HEYERES, Antoine ;  Centre Hospitalier Universitaire de Liège / ULiège > Dpt des sciences biomédicales et précliniques > Laboratoire de Biologie des Tissus Conjonctifs
Nusgens, Betty ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Laboratoire de Biologie des Tissus Conjonctifs
LE GOFF, Caroline  ;  Centre Hospitalier Universitaire de Liège - CHU > Chimie médicale
Gothot, André ;  Université de Liège - ULiège > Département des sciences cliniques > Département des sciences cliniques
Cescotto, Serge ;  Université de Liège - ULiège > Département ArGEnCo > Département ArGEnCo
Defraigne, Jean-Olivier ;  Université de Liège - ULiège > Département des sciences cliniques > Chirurgie cardio-vasculaire et thoracique
Rickert, Markus
Crielaard, Jean-Michel ;  Université de Liège - ULiège > Département des sciences de la motricité > Evaluation et entraînement des aptitudes physiques
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Language :
English
Title :
Effects of platelet-rich plasma on the healing of tendons: animal model
Publication date :
April 2012
Event name :
Biomedica 2012 - The European Life Sciences Summit
Event place :
Liège, Belgium
Event date :
18-19 April 2012
Audience :
International
Main work title :
Biomedica 2012
Pages :
180
Peer reviewed :
Peer reviewed
Available on ORBi :
since 19 May 2012

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